- ￥5600
- GeneTex
- 美国
- GTX00697
- 2025年07月11日
- WB, ICC/IF, Dot, ELISA
- Rat
- Human, Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant GST-fused human Nup98 (amino acids 1-466)
- 亚型：
IgG2c
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX00697
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
WB, ICC/IF, Dot, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
We do not recommend use of this product for Tetrahymena.
- 抗体英文名：
NUP98 antibody [2H10]
- 抗体名：
NUP98 抗体 [2H10]
- 规格：
100 μg
WB analysis of HeLa nuclear membrane extract using GTX00697 NUP98 antibody [2H10].
Dilution : 1:2000
ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10].
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
WB analysis of S. pombe cell extracts expressing wilde type Nup98 (Lane 1) or Nup98-GFP protein (Lane 2) using GTX00697 NUP98 antibody [2H10].
Dilution : 1 μg/ml
ICC/IF analysis of rat neuron cells using GTX00697 NUP98 antibody [2H10].
The dots are Nup98.
Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.
ICC/IF analysis of S. cereviciae cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
WB analysis of S. cereviciae cell extracts using GTX00697 NUP98 antibody [2H10].
Five protein bands identified by this antibody correspond to the sizes of 5 kinds of nucleoporins with multiple GFLG motifs in S. pombe.
Dilution : 2 μg/ml
ICC/IF analysis of S. pombe cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
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文献和实验Chandra B et al., Cancer Discov 2022 (PMID:34903620)
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