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文献和实验Isolation of Antigen-Specific Nanobodies
that are less accessible to/or less immunogenic for classical antibodies. Nanobodies are easily tailored for various applications. We have developed a robust technology to identify antigen-specific Nanobodies. Here, we describe, in detail, the protocols for the generation
Tracking Antigen-Specific Lymphocytes In Vivo
With the development of antigen-specific T-cell-receptor (TcR) and B-cell-receptor (BcR) transgenic (Tg) mice, it is now feasible to track single, antigen-specific lymphocyte populations throughout the course of an immune response. Adoptive
Detection of Antigen Specific B-Cells in Tissues
. For visualization and analysis of the autoreactive B-cells, the antigen of interest is selected and produced and purified from native or recombinant sources. Biotinylation of the purified antigen and subsequent use in immunohistochemistry with sections from tissue
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