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PX855载体

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  • ¥1000
  • ZYbscience
  • 中国/美国
  • ZY62887
  • 2025年07月16日
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    上海泽叶生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      PX855

    • 库存：

      20

    • 供应商：

      泽叶生物

    出品公司: ZYbscience
    载体名称: PX855
    质粒类型: CRISPR-Cas9载体；基因敲除载体；哺乳动物载体
    高拷贝/低拷贝: --
    插入位点: Age1，EcoR1
    启动子: CBh
    载体大小: 6205 bp
    5' 测序引物及序列: ctggagcacctgcctgaaatcact
    3' 测序引物及序列: GGCTGATCAGCGAGCTCTAG
    载体标签: NES、PTK2(N ter)；FRB、NES、PTK2(C ter)
    载体抗性: 氨苄青霉素
    筛选标记: --
    克隆菌株: Stbl3
    宿主细胞（系）: 常规哺乳动物细胞等
    备注: A portion of this plasmid was derived from a plasmid made by SpCas9 piece was PCR amplified of PX481 (dCas9)
    产品目录号: ZY62887
    稳定性: 瞬表达
    组成型/诱导型: 组成型
    病毒/非病毒: 非病毒

    载体质粒图谱和多克隆位点信息

    PX855载体图谱


    载体简介

    载体序列

    LOCUS       PX855	6205 bp 	DNA	SYN
DEFINITION  PX855
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="PX855"
                     /mol_type="other DNA"
     promoter        10..242
                     /label="hU6_promoter"
     misc_feature    172..191
                     /label="LKO1_5_primer"
     misc_feature    444..713
                     /label="CAG_enhancer"
     CDS             1251..3221
                     /label="ORF frame 3"
                     /translation="MLDLASLILDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRGGGGSGGGGSGGGGSEMWHEGLE
                     EASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMK
                     SGNVKDLTQAWDLYYHVFRRISKQLDLASLIL*"
     misc_feature    complement(3246..3263)
                     /label="BGH_rev_primer"
                     /translation="MLDLASLILDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRGGGGSGGGGSGGGGSEMWHEGLE
                     EASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMK
                     SGNVKDLTQAWDLYYHVFRRISKQLDLASLIL*"
     terminator      3249..3452
                     /label="bGH_PA_terminator"
                     /translation="MLDLASLILDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRGGGGSGGGGSGGGGSEMWHEGLE
                     EASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMK
                     SGNVKDLTQAWDLYYHVFRRISKQLDLASLIL*"
     rep_origin      3700..4006
                     /label="f1_origin"
                     /translation="MLDLASLILDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRGGGGSGGGGSGGGGSEMWHEGLE
                     EASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMK
                     SGNVKDLTQAWDLYYHVFRRISKQLDLASLIL*"
     misc_feature    4274..4296
                     /label="pGEX_3_primer"
                     /translation="MLDLASLILDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRGGGGSGGGGSGGGGSEMWHEGLE
                     EASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMK
                     SGNVKDLTQAWDLYYHVFRRISKQLDLASLIL*"
     promoter        4455..4483
                     /label="AmpR_promoter"
                     /translation="MLDLASLILDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRGGGGSGGGGSGGGGSEMWHEGLE
                     EASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMK
                     SGNVKDLTQAWDLYYHVFRRISKQLDLASLIL*"
     gene            4525..5385
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MLDLASLILDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRGGGGSGGGGSGGGGSEMWHEGLE
                     EASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMK
                     SGNVKDLTQAWDLYYHVFRRISKQLDLASLIL*"
     CDS             4525..5385
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5540..6159
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
   61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
  121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
  181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA
  241 CGAAACACCg gGTCTTCgaG AAGACctgtt ttagagctaG AAAtagcaag ttaaaataag
  301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcTTTTTTg ttttagagct
  361 agaaatagca agttaaaata aggctagtcc gtTTTTagcg cgtgcgccaa ttctgcagac
  421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
  481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
  541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
  601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tGtgcccagt
  661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
  721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
  781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
  841 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
  901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
  961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc
 1021 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
 1081 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
 1141 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac
 1201 ctggagcacc tgcctgaaat cacttttttt caggttGGac cggtgccacc ATGCTAGATT
 1261 TAGCTAGCTT AATCCTCGAC AAGAAGTACA GCATCGGCCT GGcCATCGGC ACCAACTCTG
 1321 TGGGCTGGGC CGTGATCACC GACGAGTACA AGGTGCCCAG CAAGAAATTC AAGGTGCTGG
 1381 GCAACACCGA CCGGCACAGC ATCAAGAAGA ACCTGATCGG AGCCCTGCTG TTCGACAGCG
 1441 GCGAAACAGC CGAGGCCACC CGGCTGAAGA GAACCGCCAG AAGAAGATAC ACCAGACGGA
 1501 AGAACCGGAT CTGCTATCTG CAAGAGATCT TCAGCAACGA GATGGCCAAG GTGGACGACA
 1561 GCTTCTTCCA CAGACTGGAA GAGTCCTTCC TGGTGGAAGA GGATAAGAAG CACGAGCGGC
 1621 ACCCCATCTT CGGCAACATC GTGGACGAGG TGGCCTACCA CGAGAAGTAC CCCACCATCT
 1681 ACCACCTGAG AAAGAAACTG GTGGACAGCA CCGACAAGGC CGACCTGCGG CTGATCTATC
 1741 TGGCCCTGGC CCACATGATC AAGTTCCGGG GCCACTTCCT GATCGAGGGC GACCTGAACC
 1801 CCGACAACAG CGACGTGGAC AAGCTGTTCA TCCAGCTGGT GCAGACCTAC AACCAGCTGT
 1861 TCGAGGAAAA CCCCATCAAC GCCAGCGGCG TGGACGCCAA GGCCATCCTG TCTGCCAGAC
 1921 TGAGCAAGAG CAGACGGCTG GAAAATCTGA TCGCCCAGCT GCCCGGCGAG AAGAAGAATG
 1981 GCCTGTTCGG AAACCTGATT GCCCTGAGCC TGGGCCTGAC CCCCAACTTC AAGAGCAACT
 2041 TCGACCTGGC CGAGGATGCC AAACTGCAGC TGAGCAAGGA CACCTACGAC GACGACCTGG
 2101 ACAACCTGCT GGCCCAGATC GGCGACCAGT ACGCCGACCT GTTTCTGGCC GCCAAGAACC
 2161 TGTCCGACGC CATCCTGCTG AGCGACATCC TGAGAGTGAA CACCGAGATC ACCAAGGCCC
 2221 CCCTGAGCGC CTCTATGATC AAGAGATACG ACGAGCACCA CCAGGACCTG ACCCTGCTGA
 2281 AAGCTCTCGT GCGGCAGCAG CTGCCTGAGA AGTACAAAGA GATTTTCTTC GACCAGAGCA
 2341 AGAACGGCTA CGCCGGCTAC ATTGACGGCG GAGCCAGCCA GGAAGAGTTC TACAAGTTCA
 2401 TCAAGCCCAT CCTGGAAAAG ATGGACGGCA CCGAGGAACT GCTCGTGAAG CTGAACAGAG
 2461 AGGACCTGCT GCGGAAGCAG CGGACCTTCG ACAACGGCAG CATCCCCCAC CAGATCCACC
 2521 TGGGAGAGCT GCACGCCATT CTGCGGCGGC AGGAAGATTT TTACCCATTC CTGAAGGACA
 2581 ACCGGGAAAA GATCGAGAAG ATCCTGACCT TCCGCATCCC CTACTACGTG GGCCCTCTGG
 2641 CCAGGGGAAA CAGCAGATTC GCCTGGATGA CCAGAAAGAG CGAGGAAACC ATCACCCCCT

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