LOCUS pgRNA-bacteria 2584 bp DNA SYNDEFINITION pgRNA-bacteriaACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..2584 /organism="pgRNA-bacteria" /mol_type="other DNA" misc_feature complement(285..302) /label="pTrcHis_rev_primer" misc_feature complement(285..302) /label="pBAD_rev_primer" terminator 335..492 /label="rrnB_terminator" terminator 458..501 /label="rrnB_T1_terminator" gene complement(1484..2344) /label="Ampicillin" /gene="Ampicillin" CDS complement(1484..2344) /label="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(2386..2414) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN 1 gAATTCTAAA GATCTTTGAC AGCTAGCTCA GTCCTAGGTA TAATACTAGT AACTTTCAGT 61 TTAGCGGTCT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC CGTTATCAAC 121 TTGAAAAAGT GGCACCGAGT CGGTGCTTTT TTTGAAGCTT GGGCCCGAAC AAAAACTCat 181 ctcagaagag gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttaaac 241 ggtctccagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa 301 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc 361 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg 421 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa 481 agactGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC TGGATCCTTA CTCGAGTCTA 541 GACTGCAGgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 601 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 661 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 721 cgtttttcca Caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 781 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 841 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 901 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 961 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1021 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1081 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1141 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 1201 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1261 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1321 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 1381 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 1441 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 1501 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 1561 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 1621 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 1681 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 1741 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 1801 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 1861 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 1921 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 1981 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 2041 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 2101 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 2161 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 2221 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 2281 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 2341 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 2401 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 2461 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 2521 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt 2581 tctg