LOCUS pYC66 5085 bp DNA SYNDEFINITION pYC66ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..5085 /organism="pYC66" /mol_type="other DNA" CDS 761..1333 /label="ORF frame 2" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" terminator 1347..1586 /label="CYC1_terminator" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" misc_feature complement(1356..1374) /label="CYC1_primer" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" misc_feature complement(1356..1374) /label="pYESTrp_rev_primer" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" misc_feature 1608..1624 /label="pBluescriptKS_primer" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" promoter complement(2287..2306) /label="T3_promoter" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" promoter complement(2323..2341) /label="M13_reverse_primer" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" misc_feature complement(2340..2362) /label="M13_pUC_rev_primer" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" promoter complement(2376..2405) /label="lac_promoter" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" gene complement(3488..4348) /label="Ampicillin" /gene="Ampicillin" /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" CDS complement(3488..4348) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(4390..4418) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcaCctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cgGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATAccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 301 agcacagact tagattggta tatatacgCA TACcataaag cacAGCTCca tagcttcaaa 361 atgtttctac tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca 421 cttcaaaaca cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt 481 taattacccg tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc 541 gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt cttgaaaatt ttttttttga 601 tttttttctc tttcgatgac ctcccattga tatttaagtt aataaacggt cttcaatttc 661 tcaagtttca gtttcatttt tcttgttcta ttacaacttt ttttacttct tgctcattag 721 aaagaaagca tagcaatcta atctaagttt tggatctaaa ATGGGCACCA CTCTTGACGA 781 CACGGCTTAC CGATACCGCA CCAGTGTCCC GGGGGACGCC GAGGCCATCG AGGCACTGGA 841 TGGGTCCTTC ACCACCGACA CCGTCTTCCG CGTCACCGCC ACCGGGGACG GCTTCACCCT 901 GCGGGAGGTG CCGGTGGACC CGCCCCTGAC CAAGGTGTTC CCCGACGACG AATCGGACGA 961 CGAATCGGAC GACGGGGAGG ACGGCGACCC TGACTCCCGG ACGTTCGTCG CGTACGGGGA 1021 CGACGGCGAC CTGGCGGGCT TCGTGGTCGT CTCGTACTCC GGCTGGAACC GCCGGCTGAC 1081 CGTCGAGGAC ATCGAGGTCG CCCCGGAGCA CCGGGGGCAC GGGGTCGGGC GCGCGTTGAT 1141 GGGGCTCGCG ACGGAGTTCG CCCGCGAGCT GGGCGCCGGG CACCTCTGGC TGGAGGTCAC 1201 CAACGTCAAC GCACCGGCGA TCCACGCGTA CCGGCGGATG GGGTTCACCC TCTGCGGCCT 1261 GGACACCGCC CTGTACGACG GCACCGCCTC GGACGGCGAG CAGGCGCTCT ACATGAGCAT 1321 GCCCTGCCCC TAAgtcgagt catgtaatta gttatgtcac gcttacattc acgccctccc 1381 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt 1441 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt 1501 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt 1561 ttgggacgct cgaaggcttt aatttgcggc cGGTACcggg ccccccctcg aggtcgacgg 1621 tatcgataag cttgatatcg aattcctgca gcccggggga tcctagggTT AATTAACGGT 1681 GAACAAAAGC TAATCTCCGA GGAAGACTTG AACGGTGAAC AAAAATTAAT CTCAGAAGAA 1741 GACTTGAACG GACTCGACGG TGAACAAAAG TTGATTTCTG AAGAAGATTT GAACGGTGAA 1801 CAAAAGCTAA TCTCCGAGGA AGACTTGAAC GGTGAACAAA AATTAATCTC AGAAGAAGAC 1861 TTGAACGGAC TCGACGGTGA ACAAAAGTTG ATTTCTGAAG AAGATTTGAA CGGTGAACAA 1921 AAGCTAATCT CCGAGGAAGA CTTGAACGGT GAACAAAAAT TAATCTCAGA AGAAGACTTG 1981 AACGGACTCG ACGGTGAACA AAAGTTGATT TCTGAAGAAG ATTTGAACGG TGAACAAAAG 2041 CTAATCTCCG AGGAAGACTT GAACGGTGAA CAAAAATTAA TCTCAGAAGA AGACTTGAAC 2101 GGACTCGACG GTGAACAAAA GTTGATTTCT GAAGAAGATT TGAACGGTGA ACAAAAGCTA 2161 ATCTCCGAGG AAGACTTGAA CGGTGAACAA AAATTAATCA ATCACtagct agtgaattcg 2221 cgccagatcc GAAGTTCCTA TACTTTctag agcggccgcc accgCGGTGG AGCTCCAGCT 2281 TTTGTTCCCT TTAGTGAGGG TTAATTGCGC GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 2341 Ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat aCgagccgga agcataaagt 2401 gtaaagcctg gggtgcctaa tgagtgagCt aactcacatt aattgcgttg cgctcactgc 2461 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 2521 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 2581 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 2641 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 2701 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 2761 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 2821 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 2881 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 2941 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 3001 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 3061 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 3121 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 3181 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 3241 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 3301 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 3361 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 3421 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 3481 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 3541 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 3601 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 3661 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 3721 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 3781 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 3841 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 3901 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 3961 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 4021 gcttttctgt gactggtgAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 4081 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 4141 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 4201 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 4261 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 4321 GGGCGACACG GAAATGTTGA ATACTCATac tcttcctttt tcaatattat tgaagcattt 4381 atcagggtta ttgtctcatg agcggataca taTTTGAATG TGGGGATCTC TAATGATGCA 4441 ATTTTTGTTT TGTACACTAA GATTCTATTT CGGAACTCTT ACCCATGTCA TTATTTactt 4501 tattttcatt attgTAATTT TTTAAGCTTT TATTATGAAT TATTTAAATA CAATTCTTTT 4561 TGATCATGTG ACAGACATCA CTGAAAGATG TGCTCTGCGT TATTAGTCAG ATTGAAATAT 4621 ACATTTTCAT GTAGATATGA TGTTCTTACT TTAAATGTTG TTCCATTTGC TAGCTTAAAT 4681 AGACGATATT ATAAATTTAA GTATTCACTA AAAATCTGAC CCATTAATGT TAGAATTAGT 4741 ATTATTTTTT GTGATATTAC ATTTTTATAT TTTGGATTTC AACCAATGCT CTGCGATATG 4801 ATTCAACAGA ATAATATGTA CAAGCTACTT TAATTCATAT ATACTAATAG ATTCACAGAT 4861 CCAGTACGAC TGCAAGAATT TACGTTTTGT TTGAAATGGT AGTAGCTACA CTAAATTAAC 4921 AATGATCGAT TAATCCACAT CCACAGTGTG TAATCATCCT AATGAAAACA AGAATAACTA 4981 CAAAAATGAA TATAAACTTT TTGCATGTTA GTCTAACCTG CTAAGAAACC ATTATTATCA 5041 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTC//