- ¥1500
- XYbscience
- 中国/美国
- XY1240
- 2025年07月06日
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- 详细信息
- 技术资料
- 保存条件：
常温
- 保质期：
三年
- 英文名：
pCOLAduet-1
- 库存：
60
- 供应商：
信裕生物
- 规格：
5ug质粒
基本信息
| 质粒类型: | 大肠杆菌表达载体（双启动子） |
|---|---|
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3719 bp (查看载体序列) |
| 5' 测序引物及序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; |
| 3' 测序引物及序列: | T7t: 5'-TGCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-His, C-S |
| 载体抗性: | Kanamycin (卡那霉素) |
| 备注: |
pRSFDuet-1 carries the RSF1030 replicon, lacI gene and kanamycin resistance gene.
|
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| XY1240 | pCOLAduet-1 | 5ug质粒 |
¥1500.00 |
质粒图谱
载体描述
pCOLADuet-1 is designed for the coexpression of two target genes from a single plasmid. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). MCS-1 encodes the six-amino acid His•Tag sequence for the creation of a N-terminal fusion and MCS2 encodes the 15 amino acid S•Tag™ peptide after the last restriction site for the creation of a C-terminal fusion if desired. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. The vector has the COLA replicon from ColA(1) and the kanamycin resistance gene. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to 8 target genes.
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