| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCAMBIA1391Z |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 11227 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | GUS、6xHis |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 潮霉素 |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pCambia1391Z
- 库存：
20
- 供应商：
泽叶生物
pCambia 1391Z载体基本信息
pCambia 1391Z载体质粒图谱和多克隆位点信息
pCambia 1391Z载体简介
pCambia 1391Z载体序列
LOCUS pCAMBIA1391Z 11227 bp ds-DNA circular SYN 01-十二月-2013
DEFINITION Agrobacterium binary vector for plant transformation, with
hygromycin- and kanamycin-resistance and LacZ-GUS genes plus the
pUC9 MCS.
ACCESSION AF234312
VERSION .
KEYWORDS pCAMBIA1391Z
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11227)
AUTHORS Cambia
TITLE Direct Submission
JOURNAL Exported 2014年6月2日 from SnapGene Viewer 1.5.3
http://www.snapgene.com
COMMENT The GenBank record was corrected by inserting a G at position 4743.
FEATURES Location/Qualifiers
source 1..11227
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
CDS join(2..16,207..2024)
/codon_start=1
/product="beta-glucuronidase"
/label="GUS"
/label="This version of the gusA gene has a 5' extension
with a catalase intron to ensure expression in plants but
not bacteria."
/protein_id="
"
intron 17..206
/label="cat1 intron"
/label="castor bean catalase intron, modified"
CDS 2031..2048
/codon_start=1
/product="6xHis affinity tag"
/label="6xHis"
/translation="HHHHHH"
terminator 2083..2335
/label="NOS terminator"
/label="nopaline synthase terminator and poly(A) signal"
misc_feature 2357..2381
/label="RB T-DNA repeat"
/label="right border repeat from nopaline C58 T-DNA"
CDS 3681..4310
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/label="pVS1 StaA"
CDS 4739..5812
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/label="pVS1 RepA"
/label="
"
/protein_id="
"
misc_feature 6416..6556
/label="bom"
/label="basis of mobility region from pBR322"
rep_origin complement(6742..7330)
/direction=LEFT
/label="ori"
/label="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7417..8211)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label="KanR"
/label="confers resistance to kanamycin"
/protein_id="
"
misc_feature 8636..8660
/label="LB T-DNA repeat"
/label="left border repeat from nopaline C58 T-DNA"
polyA_signal 8738..8912
/label="CaMV poly(A) signal"
/label="cauliflower mosaic virus polyadenylation signal"
CDS complement(8952..9977)
/codon_start=1
/product="hygromycin B phosphotransferase"
/label="HygR"
/label="confers resistance to hygromycin"
promoter complement(10045..10722)
/label="CaMV 35S promoter (enhanced)"
/label="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
promoter 10936..10966
/label="lac promoter"
/label="promoter for the E. coli lac operon"
protein_bind 10974..10990
/bound_moiety="lac repressor encoded by lacI"
/label="lac operator"
/label="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 10998..11014
/label="M13 rev"
/label="common sequencing primer, one of multiple similar
variants"
CDS 11010..11207
/codon_start=1
/gene="lacZ (fragment)"
/product="LacZ-alpha fragment of?beta-galactosidase"
/label="lacZ-alpha"
/protein_id="
"
/translation="MTMITPSLAAGRRIPGNSLAVVLQRRDWENPGVTQLNRLAAHPPF
ASWRNSEEARTDRPSQQLRSL"
misc_feature 11027..11062
/label="MCS"
/label="multiple cloning site from pUC9"
primer_bind complement(11063..11079)
/label="M13 fwd"
/label="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
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pCambia1391Z载体
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