- ¥1000
- XYbscience
- 中国/美国
- XY2040
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
三年
- 英文名：
pAd/CMV/V5-DEST
- 库存：
60
- 供应商：
信裕生物
- 规格：
5ug质粒
基本信息
| 质粒类型: | 腺病毒载体 |
|---|---|
| 启动子: | CMV |
| 克隆方法: | Gateway |
| 载体大小: | 36686 bp (查看载体序列) |
| 5' 测序引物及序列: | CMVPro Fwd: 5'd[CGCAAATGGGCGGTAGGCGTG]3' |
| 载体标签: | V5 |
| 载体抗性: | Ampicillin (氨苄青霉素) |
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| XY2040 | pAd/CMV/V5-DEST | 5ug质粒 |
¥1000.00 |
质粒图谱
载体描述
The pAd⁄CMV⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄CMV⁄V5-DEST™ vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter.
Advantages
• High efficiency and rapid recombination cloning
• Produces high titer adenoviral stocks
• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
• Produces replication-incompetent virus for enhanced biosafety of the system
• Amenable for use in high-throughput applications
Key Features
• Gateway® Technology for efficient and rapid cloning
• CMV promoter for high-level constitutive expression of gene of interest
• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the
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文献和实验a look from the following website just by clicking "Tyrosine Phosphorylation and Dimerization" http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-43F85YV-2&_user=1111158&_coverDate=06%2F29%2F2001&_rdoc=1&_fmt=full&_orig=search
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
-818.2009.03142.x (2009). 6. Hutson, M. S., & Ma, X. Plasma and cavitation dynamics during pulsed laser microsurgery in vivo . Physical review letters. 99, 158104 (2007). 7. Venugopalan, V., Guerra, A., 3rd, Nahen, K., & Vogel
的方法。 靶点验证 1、pTYNE-53 验证载体构建 为了验证基因敲除靶点的效率，我们把 TP53 第四外显子部分序列通过 XhoI、HindIII 酶切位点连接到 pTYNE 载体，通过 pTYNE 载体验证靶点的切割效率。构建完成的载体命名为 pTYNE-53。 构建完成后 pTYNE-53 载体序列： 注： 黄色：CMV promoter；灰色：TP53 第四外显子部分序列；绿色：EGFP 序列；下划线：CRISPR 靶点
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