pTRIPZ载体

LOCUS       pTRIPZ	13320 bp 	DNA     circular	SYN
DEFINITION  pTRIPZ
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="pTRIPZ"
                     /mol_type="other DNA"
     misc_feature    454..634
                     /label="HIV-1_5_LTR"
     misc_feature    454..634
                     /label="truncHIV-1_3_LTR"
     misc_feature    745..789
                     /label="HIV-1_psi_pack"
     CDS             complement(1015..1476)
                     /label="ORF frame 1"
     CDS             1181..2068
                     /label="ORF frame 2"
     misc_feature    1303..1536
                     /label="RRE"
     misc_feature    2067..2082
                     /label="cPPT"
     misc_feature    complement(2245..2619)
                     /label="sh_ble"
     gene            complement(2248..2619)
                     /label="bleo"
                     /gene="bleo"
     promoter        complement(2629..2647)
                     /label="T7_promoter"
     CDS             2744..3700
                     /label="ORF frame 2"
     misc_feature    2963..2982
                     /label="pCEP_fwd_primer"
     misc_feature    2965..2989
                     /label="LNCX_primer"
     promoter        complement(4039..4057)
                     /label="T7_promoter"
     promoter        4077..5281
                     /label="hUbC_promoter"
     misc_feature    complement(5294..5327)
                     /label="loxP"
     misc_feature    5294..5327
                     /label="loxP"
     CDS             5334..6041
                     /label="ORF frame 3"
     misc_feature    complement(6042..6075)
                     /label="loxP"
     misc_feature    6042..6075
                     /label="loxP"
     misc_feature    6097..6660
                     /label="IRES"
     CDS             6673..7275
                     /label="ORF frame 1"
     gene            6688..7275
                     /label="puro (variant)"
                     /gene="puro (variant)"
     misc_feature    7293..7868
                     /label="WPRE"
     CDS             7381..7908
                     /label="ORF frame 1"
     misc_feature    8086..8138
                     /label="delta_U3"
     misc_feature    8139..8319
                     /label="HIV-1_5_LTR"
     misc_feature    8139..8319
                     /label="truncHIV-1_3_LTR"
     misc_feature    complement(8420..8437)
                     /label="BGH_rev_primer"
     terminator      8423..8650
                     /label="bGH_PA_terminator"
     rep_origin      8713..9019
                     /label="f1_origin"
     misc_feature    complement(9133..9153)
                     /label="pBABE_3_primer"
     misc_feature    complement(9139..9354)
                     /label="SV40_enhancer"
     promoter        9151..9419
                     /label="SV40_promoter"
     rep_origin      9318..9395
                     /label="SV40_origin"
     misc_feature    9380..9399
                     /label="SV40pro_F_primer"
     gene            9531..10542
                     /label="hygroB"
                     /gene="hygroB"
     CDS             complement(9625..10278)
                     /label="ORF frame 1"
     CDS             9874..10545
                     /label="ORF frame 1"
     terminator      10678..10797
                     /label="SV40_PA_terminator"
     misc_feature    10766..10785
                     /label="EBV_rev_primer"
     promoter        complement(10841..10859)
                     /label="M13_reverse_primer"
     misc_feature    complement(10858..10880)
                     /label="M13_pUC_rev_primer"
     promoter        complement(10894..10923)
                     /label="lac_promoter"
     gene            complement(12006..12866)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(12006..12866)
                     /label="ORF frame 2"
     promoter        complement(12908..12936)
                     /label="AmpR_promoter"
     terminator      13022..13153
                     /label="SV40_PA_terminator"
     misc_feature    13110..13129
                     /label="EBV_rev_primer"
ORIGIN
    1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
   61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
  121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
  181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
  241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
  301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
  361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
  421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
  481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
  541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
  601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
  661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
  721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
  781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
  841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
  901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
  961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
 1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
 2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
 2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
 2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
 2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
 2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
 2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
 2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
 2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
 2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
 2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
 2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
 2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
 2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
 2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
 2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
 2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtcgc
 3001 caccatgagc gagctgatca aggagaacat gcacatgaag ctgtacatgg agggcaccgt
 3061 gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
 3121 gaccatgaag atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
 3181 taccagcttc atgtacggca gcaaagcctt catcaaccac acccagggca tccccgactt
 3241 ctttaagcag tccttccctg agggcttcac atgggagaga atcaccacat acgaagacgg
 3301 gggcgtgctg accgctaccc aggacaccag cttccagaac ggctgcatca tctacaacgt
 3361 caagatcaac ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacacgcgg
 3421 ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc ggcctgagag gccacagcca
 3481 gatggccctg aagctcgtgg gcgggggcta cctgcactgc tccttcaaga ccacatacag
 3541 atccaagaaa cccgctaaga acctcaagat gcccggcttc cacttcgtgg accacagact
 3601 ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgaga tggctgtggc
 3661 caagtactgc gacctcccta gcaaactggg gcacagataa tcgatagttt gtttgaatga
 3721 ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc tgggattact
 3781 tcttcaggtt aacccaacag aaggctcgag gtaaccggat cctgatcaga attcaagggg
 3841 ctactttagg agcaattatc ttgtttacta aaactgaata ccttgctatc tctttgatac
 3901 atttttacaa agctgaatta aaatggtata aattaaatca cttttttcaa ttggaagact
 3961 aatgcggccg gccattactc cgtctcgtgt cttgttgcat atgtctgctg gtttgtttga
 4021 tgttgtttgc gggcgggccc tatagtgagt cgtattacct aggacgcgtg gcctccgcgc
 4081 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
 4141 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
 4201 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
 4261 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
 4321 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag
 4381 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg
 4441 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt
 4501 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta