| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | FUW-tetO-hOCT4 |
| 质粒类型: | 慢病毒载体；四环素调控载体；Tet-On系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | EcoRI |
| 启动子: | TRE/Minimal CMV |
| 载体大小: | 9477 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | bleomycin, phleomycin, and Zeocin? |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 大部分哺乳动物细胞 |
| 备注: | FUW-tetO-hOCT4 vector expressing the reverse tetracycline transactivator |
| 产品目录号: | ZY20726 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 慢病毒 |
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- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
FUW-tetO-hOCT4
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FUW-tetO-hOCT4 9477 bp DNA SYN
DEFINITION FUW-tetO-hOCT4
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..9477
/organism="FUW-tetO-hOCT4"
/mol_type="other DNA"
promoter 28..604
/label="CMV_immearly_promoter"
misc_feature 107..394
/label="CAG_enhancer"
misc_feature 561..581
/label="CMV_fwd_primer"
misc_feature 624..804
/label="truncHIV-1_3_LTR"
misc_feature 624..804
/label="HIV-1_5_LTR"
misc_feature 915..959
/label="HIV-1_psi_pack"
CDS 1347..2234
/label="ORF frame 3"
misc_feature 1469..1702
/label="RRE"
misc_feature 2233..2248
/label="cPPT"
misc_feature 2391..2708
/label="TRE"
promoter 2709..2828
/label="CMV2_promoter"
misc_feature 2740..2759
/label="pCEP_fwd_primer"
misc_feature 2742..2766
/label="LNCX_primer"
CDS 2851..3933
/label="ORF frame 1"
misc_feature 3960..4547
/label="WPRE"
CDS 4048..5097
/label="ORF frame 1"
CDS 4061..4573
/label="ORF frame 2"
misc_feature complement(4550..4566)
/label="pBluescriptKS_primer"
misc_feature 4734..4749
/label="cPPT"
misc_feature 4734..4755
/label="U3PPT"
misc_feature 5071..5251
/label="HIV-1_5_LTR"
misc_feature 5071..5251
/label="truncHIV-1_3_LTR"
misc_feature complement(5277..5294)
/label="BGH_rev_primer"
terminator 5280..5507
/label="bGH_PA_terminator"
rep_origin 5570..5876
/label="f1_origin"
misc_feature complement(5990..6010)
/label="pBABE_3_primer"
misc_feature complement(5996..6211)
/label="SV40_enhancer"
promoter 6008..6276
/label="SV40_promoter"
rep_origin 6175..6252
/label="SV40_origin"
misc_feature 6237..6256
/label="SV40pro_F_primer"
promoter 6370..6437
/label="EM7_promoter"
misc_feature 6438..6812
/label="sh_ble"
gene 6438..6809
/label="bleo"
/gene="bleo"
terminator 6945..7064
/label="SV40_PA_terminator"
misc_feature 7033..7052
/label="EBV_rev_primer"
promoter complement(7108..7126)
/label="M13_reverse_primer"
misc_feature complement(7125..7147)
/label="M13_pUC_rev_primer"
promoter complement(7161..7190)
/label="lac_promoter"
gene complement(8273..9133)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(8273..9133)
/label="ORF frame 3"
promoter complement(9175..9203)
/label="AmpR_promoter"
ORIGIN
1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttaatgat ctcgagttta
2401 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
2461 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
2521 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
2581 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
2641 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc
2701 gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt
2761 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
2821 tccagcctcc gcggccccga attcgccacc atggcgggac acctggcttc agattttgcc
2881 ttctcgcccc ctccaggtgg tggaggtgat gggccagggg ggccggagcc gggctgggtt
2941 gatcctcgga cctggctaag cttccaaggc cctcctggag ggccaggaat cgggccgggg
3001 gttgggccag gctctgaggt gtgggggatt cccccatgcc ccccgccgta tgagttctgt
3061 ggggggatgg cgtactgtgg gccccaggtt ggagtggggc tagtgcccca aggcggcttg
3121 gagacctctc agcctgaggg cgaagcagga gtcggggtgg agagcaactc cgatggggcc
3181 tccccggagc cctgcaccgt cacccctggt gccgtgaagc tggagaagga gaagctggag
3241 caaaacccgg aggagtccca ggacatcaaa gctctgcaga aagaactcga gcaatttgcc
3301 aagctcctga agcagaagag gatcaccctg ggatatacac aggccgatgt ggggctcacc
3361 ctgggggttc tatttgggaa ggtattcagc caaacgacca tctgccgctt tgaggctctg
3421 cagcttagct tcaagaacat gtgtaagctg cggcccttgc tgcagaagtg ggtggaggaa
3481 gctgacaaca atgaaaatct tcaggagata tgcaaagcag aaaccctcgt gcaggcccga
3541 aagagaaagc gaaccagtat cgagaaccga gtgagaggca acctggagaa tttgttcctg
3601 cagtgcccga aacccacact gcagcagatc agccacatcg cccagcagct tgggctcgag
3661 aaggatgtgg tccgagtgtg gttctgtaac cggcgccaga agggcaagcg atcaagcagc
3721 gactatgcac aacgagagga ttttgaggct gctgggtctc ctttctcagg gggaccagtg
3781 tcctttcctc tggccccagg gccccatttt ggtaccccag gctatgggag ccctcacttc
3841 actgcactgt actcctcggt ccctttccct gagggggaag cctttccccc tgtctctgtc
3901 accactctgg gctctcccat gcattcaaac tgagaattcg atatcaagct tatcgataat
3961 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
4021 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
4081 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
4141 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
4201 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
4261 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
4321 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
4381 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
4441 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
4501 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
4561 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
4621 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
4681 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
4741 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
4801 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
4861 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
4921 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
4981 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
5041 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
5101 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
5161 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
5221 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
5281 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
5341 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
5401 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
5461 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
5521 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
5581 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
5641 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
5701 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
5761 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
5821 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
5881 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
5941 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
6001 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
6061 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
6121 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
6181 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
6241 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
6301 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
6361 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
6421 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
6481 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
6541 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
6601 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
6661 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
6721 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
6781 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
6841 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
6901 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
6961 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
7021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
7081 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
7141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
7201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
7261 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
7321 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
7381 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
7441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
7501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
7561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
7621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
7681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
7741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
7801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
7861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
7921 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
7981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
8041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
8101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
8161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
8221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
8281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
8341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
8401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
8461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
8521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
8581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
8641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
8701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
8761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
8821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
8881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
8941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
9001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
9061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
9121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
9181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
9241 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
9301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
9361 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
9421 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcg
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)可以受四环素或者其衍生物的调节而发生构象变化，从而改变与相应的DNA序列(四环素抗性元件，Tet resistance operon，TetO)的结合能力。tTA或rtTA的另一个功能就是转录激活功能，当其在真核细胞内与相应的DNA反应元件结合后可以启动下游基因的转录。rtTA与tTA相比有几个氨基酸位置的突变，突变的结果是两者对四环素反应后产生的效应完全相反。rtTA只有在四环素存在的情况下才能与反应元件TetO相结合，而tTA只有在四环素不存在的情况下才能与TetO结合。在具体的应用中，tTA
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FUW-tetO-hOCT4载体
¥900
