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Tet-pLKO-puro载体

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  • ZYbscience
  • 中国/美国
  • ZY
  • 2025年07月10日
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    上海泽叶生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      Tet-pLKO-puro

    • 库存：

      20

    • 供应商：

      泽叶生物

    载体基本信息

    出品人: ZYbscience
    载体名称: Tet-pLKO-puro
    质粒类型: 慢病毒载体；RNAi载体；四环素诱导载体；Tet-On载体
    高拷贝/低拷贝: 低拷贝
    克隆方法: AgeI；EcoRI
    启动子: CMV
    载体大小: 10633 bp
    5' 测序引物及序列: 5’ GGCAGGGATATTCACCATTATCGTTTCAGA 3’
    3' 测序引物及序列: --
    载体标签: --
    载体抗性: 氨苄青霉素
    筛选标记: Puromycin
    克隆菌株: Stbl3 感受态细胞 ，以维持质粒的稳定性。
    宿主细胞（系）: 哺乳动物细胞
    备注:
    其它筛选标记 Tet-pLKO-neo
    产品目录号: ZY21915
    稳定性: 稳表达
    组成型/诱导型: 诱导型
    病毒/非病毒: 慢病毒

    载体质粒图谱和多克隆位点信息

    Tet-pLKO-puro载体图谱

    载体简介

    载体序列

    LOCUS       Tet-pLKO-puro	10633 bp 	DNA	SYN
DEFINITION  Tet-pLKO-puro
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Tet-pLKO-puro"
                     /mol_type="other DNA"
     misc_feature    516..1088
                     /label="bGlob_int"
     misc_feature    1096..1115
                     /label="pCAG_F_primer"
     promoter        1143..1161
                     /label="T7_promoter"
     CDS             1172..1819
                     /label="ORF frame 2"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSGSREF*"
     misc_feature    1175..1786
                     /label="TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSGSREF*"
     misc_feature    1826..2389
                     /label="IRES"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSGSREF*"
     CDS             complement(2315..3070)
                     /label="ORF frame 1"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWILIIVFFKGKPRPRGSGGLDVFLTSTKHM*"
     gene            2420..3019
                     /label="puro (variant)"
                     /gene="puro (variant)"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWILIIVFFKGKPRPRGSGGLDVFLTSTKHM*"
     CDS             2420..3019
                     /label="ORF frame 2"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3130..3151
                     /label="U3PPT"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3130..3145
                     /label="cPPT"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3147..3199
                     /label="delta_U3"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3200..3380
                     /label="HIV-1_5_LTR"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3200..3380
                     /label="truncHIV-1_3_LTR"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     terminator      3455..3574
                     /label="SV40_PA_terminator"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3543..3562
                     /label="EBV_rev_primer"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     rep_origin      3592..3690
                     /label="SV40_origin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3675..3694
                     /label="SV40pro_F_primer"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     promoter        complement(3769..3787)
                     /label="T7_promoter"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     promoter        complement(3797..3813)
                     /label="M13_forward20_primer"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    complement(3806..3828)
                     /label="M13_pUC_fwd_primer"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3806..3954
                     /label="lacZ_a"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     rep_origin      3972..4278
                     /label="f1_origin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     promoter        4471..4499
                     /label="AmpR_promoter"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     gene            4541..5401
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     CDS             4541..5401
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5556..6175
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6484..6513
                     /label="lac_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6527..6549
                     /label="M13_pUC_rev_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6548..6566
                     /label="M13_reverse_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6583..6602
                     /label="T3_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6856..7036
                     /label="truncHIV-1_3_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6856..7036
                     /label="HIV-1_5_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    7147..7191
                     /label="HIV-1_psi_pack"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             7579..8436
                     /label="ORF frame 1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    7701..7934
                     /label="RRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    8463..8482
                     /label="H1_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    10468..10483
                     /label="cPPT"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
ORIGIN
    1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
   61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
  121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
  181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
  241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
  301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
  361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
  421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
  481 gaccgaatca ccgacctctc tccccagggg gatctgtgag tttggggacc cttgattgtt
  541 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
  601 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
  661 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
  721 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
  781 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
  841 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
  901 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc
  961 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
 1021 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt
 1081 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
 1141 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat
 1201 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
 1261 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
 1321 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
 1381 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
 1441 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
 1501 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
 1561 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
 1621 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
 1681 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
 1741 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag
 1801 cggctcccgg gagttctagg gatctgcccc tctccctccc ccccccctaa cgttactggc
 1861 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
 1921 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
 1981 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
 2041 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
 2101 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
 2161 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
 2221 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
 2281 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
 2341 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
 2401 ggatccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
 2461 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
 2521 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
 2581 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
 2641 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
 2701 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
 2761 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
 2821 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
 2881 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
 2941 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
 3001 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
 3061 acgaccccat gcatcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
 3121 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
 3181 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
 3241 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
 3301 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
 3361 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
 3421 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
 3481 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
 3541 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
 3601 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
 3661 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
 3721 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
 3781 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
 3841 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
 3901 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
 3961 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
 4021 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
 4081 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
 4141 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
 4201 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
 4261 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
 4321 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
 4381 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
 4441 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
 4501 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
 4561 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
 4621 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
 4681 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
 4741 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
 4801 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
 4861 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
 4921 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
 4981 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
 5041 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
 5101 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
 5161 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
 5221 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
 5281 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
 5341 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
 5401 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
 5461 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
 5521 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
 5581 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
 5641 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
 5701 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
 5761 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
 5821 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
 5881 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
 5941 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
 6001 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
 6061 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
 6121 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
 6181 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
 6241 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
 6301 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
 6361 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
 6421 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
 6481 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
 6541 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
 6601 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
 6661 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
 6721 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
 6781 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
 6841 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
 6901 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
 6961 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
 7021 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
 7081 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
 7141 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
 7201 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
 7261 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
 7321 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
 7381 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
 7441 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
 7501 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
 7561 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
 7621 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
 7681 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
 7741 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
 7801 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
 7861 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
 7921 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
 7981 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
 8041 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
 8101 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
 8161 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
 8221 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
 8281 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
 8341 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
 8401 gtgaacggat ctcgacggta tcgatcacga gactagcctc gagcggccgc aatatttgca
 8461 tgtcgctatg tgttctggga aatcaccata aacgtgaaat ccctatcagt gatagagact
 8521 tataagttcc ctatcagtga tagagacacc ggtgtgttgt aaatgagcac acaaaataca
 8581 catgctaaaa tattatattc tatgaccttt ataaaatcaa ccaaaatctt ctttttaata
 8641 actttagtat caataattag aatttttatg ttcctttttg caaactttta ataaaaatga
 8701 gcaaaataaa aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct ttaataatag
 8761 ctactccacc acttgttcct aagcggtcag ctcctgcttc aatcattttt tgagcatctt
 8821 caaatgttct aactccacca gctgctttaa ctaaagcatt gtctttaaca actgacttca
 8881 ttagtttaac atcttcaaat gttgcacctg attttgaaaa tcctgttgat gttttaacaa
 8941 attctaatcc agcttcaaca gctatttcac aagctttcat gatttcttct tttgttaata
 9001 aacaattttc cataatacat ttaacaacat gtgatccagc tgcttttttt acagctttca
 9061 tgtcttctaa aactaattca taatttttgt cttttaatgc accaatattt aataccatat
 9121 caatttctgt tgcaccatct ttaattgctt cagaaacttc gaatgctttt gtagctgttg
 9181 tgcatgcacc tagaggaaaa cctacaacat ttgttattcc tacatttgtg ccttttaata
 9241 attctttaca atagcttgtt caatatgaat taacacaaac tgttgcaaaa tcaaattcaa
 9301 ttgcttcatc acataattgt ttaatttcag ctttcgtagc atcttgtttt aataatgtgt
 9361 gatctatata tttgtttagt ttcatttttt ctcctatata ttcattttta attttaattc
 9421 tttaataatt tcgtctactt taactttagc gttttgaaca gattcaccaa cacctataaa
 9481 ataaattttt agtttaggtt cagttccact tgggcgaaca gcaaatcatg acttatcttc
 9541 taaataaaat tttagtaagt cttgtcctgg catattatac attccatcga tgtagtcttc
 9601 aacattaaca actttaagtc cagcaatttg agttaagggt gttgctctca atgatttcat
 9661 taatggttca atttttaatt tcttttcttc tggtttaaaa ttcaagttta aagtgaaagt
 9721 gtaatatgca cccatttctt taaataaatc ttctaaatag tctactaatg ttttattttg
 9781 ttttttataa aatcaagcag cctctgctat taatatagaa gcttgtattc catctttatc
 9841 tctagctgag tcatcaatta catatccata actttcttca taagcaaaaa caaaatttaa
 9901 tccgttatct tcttctttag caatttctct acccattcat ttaaatccag ttaaagtttt
 9961 tacaatatta actccatatt tttcatgagc gattctatca cccaaatcac ttgttacaaa
10021 acttgaatat agagccggat tttttggaat gctatttaag cgttttagat ttgataattt
10081 tcaatcaatt aaaattggtc ctgtttgatt tccatctaat cttacaaaat gaccatcatg
10141 ttttattgcc attccaaatc tgtcagcatc tgggtcattc ataataataa tatctgcatc
10201 atgtttaata ccatattcaa gcggtatttt tcatgcagga tcaaattctg gatttggatt
10261 tacaacattt ttaaatgttt catcttcaaa tgcatgctct tcaacctcaa taacgttata
10321 tcctgattca cgtaatattt ttggggtaaa tttagttcct gttccattaa ctgcgctaaa
10381 aataattttt aaatcttttt tagcttcttg ctcttttttg tagaattctc gacctcgaga
10441 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
10501 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
10561 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
10621 cggctcgagg ggg
//

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