pLKO.1-TRC载体

pLKO.1-TRC是pLKO.1 puro载体在AgeI和EcoRI限制性内切酶位点中间插入有片段情况下的质粒，可以用来替代pLKO.1 puro载体。

LOCUS       pLKO.1 - TRC cloning vector	8901 bp 	DNA   circular	SYN
DEFINITION  pLKO.1 - TRC cloning vector
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="pLKO.1 - TRC cloning vector"
                     /mol_type="other DNA"
     CDS             289..1128
                     /label="ORF frame 1"
     CDS             complement(334..1179)
                     /label="ORF frame 1"
     gene            529..1128
                     /label="puro (variant)"
                     /gene="puro (variant)"
     misc_feature    1239..1254
                     /label="cPPT"
     misc_feature    1239..1260
                     /label="U3PPT"
     misc_feature    1256..1308
                     /label="delta_U3"
     misc_feature    1309..1489
                     /label="HIV-1_5_LTR"
     misc_feature    1309..1489
                     /label="truncHIV-1_3_LTR"
     terminator      1564..1683
                     /label="SV40_PA_terminator"
     misc_feature    1652..1671
                     /label="EBV_rev_primer"
     rep_origin      1701..1799
                     /label="SV40_origin"
     misc_feature    1784..1803
                     /label="SV40pro_F_primer"
     promoter        complement(1878..1896)
                     /label="T7_promoter"
     promoter        complement(1906..1922)
                     /label="M13_forward20_primer"
     misc_feature    complement(1915..1937)
                     /label="M13_pUC_fwd_primer"
     misc_feature    1915..2063
                     /label="lacZ_a"
     rep_origin      2081..2387
                     /label="f1_origin"
     promoter        2580..2608
                     /label="AmpR_promoter"
     gene            2650..3510
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             2650..3510
                     /label="ORF frame 1"
     rep_origin      3665..4284
                     /label="pBR322_origin"
     promoter        4593..4622
                     /label="lac_promoter"
     misc_feature    4636..4658
                     /label="M13_pUC_rev_primer"
     promoter        4657..4675
                     /label="M13_reverse_primer"
     promoter        4692..4711
                     /label="T3_promoter"
     misc_feature    4965..5145
                     /label="HIV-1_5_LTR"
     misc_feature    4965..5145
                     /label="truncHIV-1_3_LTR"
     misc_feature    5256..5300
                     /label="HIV-1_psi_pack"
     CDS             5688..6545
                     /label="ORF frame 3"
     misc_feature    5810..6043
                     /label="RRE"
     promoter        6579..6811
                     /label="hU6_promoter"
     misc_feature    6741..6760
                     /label="LKO1_5_primer"
     misc_feature    8736..8751
                     /label="cPPT"
     CDS             complement(8817..530)
                     /label="ORF frame 2"
ORIGIN
    1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
   61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
  121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
  181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
  241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
  301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
  361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
  421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
  481 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac
  541 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
  601 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
  661 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
  721 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
  781 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
  841 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
  901 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
  961 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
 1021 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
 1081 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
 1141 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca
 1201 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
 1261 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
 1321 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
 1381 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
 1441 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
 1501 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
 1561 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
 1621 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
 1681 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
 1741 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
 1801 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
 1861 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
 1921 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
 1981 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
 2041 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
 2101 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
 2161 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
 2221 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
 2281 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
 2341 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
 2401 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
 2461 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
 2521 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
 2581 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
 2641 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
 2701 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
 2761 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
 2821 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
 2881 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
 2941 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
 3001 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
 3061 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
 3121 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
 3181 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
 3241 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
 3301 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
 3361 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
 3421 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
 3481 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
 3541 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
 3601 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
 3661 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
 3721 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
 3781 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
 3841 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
 3901 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
 3961 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
 4021 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
 4081 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
 4141 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
 4201 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
 4261 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
 4321 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
 4381 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
 4441 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
 4501 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
 4561 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
 4621 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
 4681 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
 4741 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
 4801 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
 4861 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
 4921 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
 4981 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
 5041 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
 5101 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
 5161 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
 5221 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
 5281 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
 5341 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
 5401 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
 5461 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
 5521 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
 5581 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
 5641 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
 5701 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
 5761 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
 5821 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
 5881 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
 5941 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
 6001 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
 6061 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
 6121 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
 6181 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
 6241 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
 6301 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
 6361 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
 6421 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
 6481 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag
 6541 actagcctcg agcggccgcc cccttcaccg agggcctatt tcccatgatt ccttcatatt
 6601 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
 6661 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
 6721 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
 6781 tcttggcttt atatatcttg tggaaaggac gaaacACCGG TGTGTTGTAA ATGAGCACAC
 6841 AAAATACACA TGCTAAAATA TTATATTCTA TGACCTTTAT AAAATCAACC AAAATCTTCT
 6901 TTTTAATAAC TTTAGTATCA ATAATTAGAA TTTTTATGTT CCTTTTTGCA AACTTTTAAT
 6961 AAAAATGAGC AAAATAAAAA AACGCTAGTT TTAGTAACTC GCGTTGTTTT CTTCACCTTT
 7021 AATAATAGCT ACTCCACCAC TTGTTCCTAA GCGGTCAGCT CCTGCTTCAA TCATTTTTTG
 7081 AGCATCTTCA AATGTTCTAA CTCCACCAGC TGCTTTAACT AAAGCATTGT CTTTAACAAC
 7141 TGACTTCATT AGTTTAACAT CTTCAAATGT TGCACCTGAT TTTGAAAATC CTGTTGATGT
 7201 TTTAACAAAT TCTAATCCAG CTTCAACAGC TATTTCACAA GCTTTCATGA TTTCTTCTTT
 7261 TGTTAATAAA CAATTTTCCA TAATACATTT AACAACATGT GATCCAGCTG CTTTTTTTAC
 7321 AGCTTTCATG TCTTCTAAAA CTAATTCATA ATTTTTGTCT TTTAATGCAC CAATATTTAA
 7381 TACCATATCA ATTTCTGTTG CACCATCTTT AATTGCTTCA GAAACTTCGA ATGCTTTTGT
 7441 AGCTGTTGTG CATGCACCTA GAGGAAAACC TACAACATTT GTTATTCCTA CATTTGTGCC
 7501 TTTTAATAAT TCTTTACAAT AGCTTGTTCA ATATGAATTA ACACAAACTG TTGCAAAATC
 7561 AAATTCAATT GCTTCATCAC ATAATTGTTT AATTTCAGCT TTCGTAGCAT CTTGTTTTAA
 7621 TAATGTGTGA TCTATATATT TGTTTAGTTT CATTTTTTCT CCTATATATT CATTTTTAAT
 7681 TTTAATTCTT TAATAATTTC GTCTACTTTA ACTTTAGCGT TTTGAACAGA TTCACCAACA
 7741 CCTATAAAAT AAATTTTTAG TTTAGGTTCA GTTCCACTTG GGCGAACAGC AAATCATGAC
 7801 TTATCTTCTA AATAAAATTT TAGTAAGTCT TGTCCTGGCA TATTATACAT TCCATCGATG
 7861 TAGTCTTCAA CATTAACAAC TTTAAGTCCA GCAATTTGAG TTAAGGGTGT TGCTCTCAAT
 7921 GATTTCATTA ATGGTTCAAT TTTTAATTTC TTTTCTTCTG GTTTAAAATT CAAGTTTAAA
 7981 GTGAAAGTGT AATATGCACC CATTTCTTTA AATAAATCTT CTAAATAGTC TACTAATGTT
 8041 TTATTTTGTT TTTTATAAAA TCAAGCAGCC TCTGCTATTA ATATAGAAGC TTGTATTCCA
 8101 TCTTTATCTC TAGCTGAGTC ATCAATTACA TATCCATAAC TTTCTTCATA AGCAAAAACA
 8161 AAATTTAATC CGTTATCTTC TTCTTTAGCA ATTTCTCTAC CCATTCATTT AAATCCAGTT
 8221 AAAGTTTTTA CAATATTAAC TCCATATTTT TCATGAGCGA TTCTATCACC CAAATCACTT
 8281 GTTACAAAAC TTGAATATAG AGCCGGATTT TTTGGAATGC TATTTAAGCG TTTTAGATTT
 8341 GATAATTTTC AATCAATTAA AATTGGTCCT GTTTGATTTC CATCTAATCT TACAAAATGA
 8401 CCATCATGTT TTATTGCCAT TCCAAATCTG TCAGCATCTG GGTCATTCAT AATAATAATA
 8461 TCTGCATCAT GTTTAATACC ATATTCAAGC GGTATTTTTC ATGCAGGATC AAATTCTGGA
 8521 TTTGGATTTA CAACATTTTT AAATGTTTCA TCTTCAAATG CATGCTCTTC AACCTCAATA
 8581 ACGTTATATC CTGATTCACG TAATATTTTT GGGGTAAATT TAGTTCCTGT TCCATTAACT
 8641 GCGCTAAAAA TAATTTTTAA ATCTTTTTTA GCTTCTTGCT CTTTTTTGTA GAATTCtcga
 8701 cctcgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
 8761 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
 8821 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
 8881 tttggccgcg gctcgagggg g
//