pCMV-BD载体

pCMV-BD载体

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  • ZYbscience
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  • 2025年07月16日
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    • 详细信息
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      pCMV-BD

    • 库存：

      20

    • 供应商：

      泽叶生物

    pCMV BD载体基本信息

    出品公司:
    ZYbscience
    载体名称: pCMV-BD
    质粒类型: 哺乳细胞杂交系统载体
    高拷贝/低拷贝: 高拷贝
    启动子: CMV
    克隆方法: 多克隆位点，限制性内切酶
    载体大小: 4730bp
    5' 测序引物及序列: Gal4-N-term (N), T7 (C)
    3' 测序引物及序列: --
    载体标签: Gal4-DBD
    载体抗性: Kanamycin
    筛选标记: --
    备注:
    产品目录号:
    稳定性: --
    组成型: --
    病毒/非病毒: --

    pCMV BD载体质粒图谱和多克隆位点信息

    pCMV-BD载体图谱



    pCMV-BD多克隆位点

    pCMV BD载体简介

    pCMV BD载体序列

    LOCUS       pCMV-BD	4730 bp 	DNA	SYN
DEFINITION  pCMV-BD
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="pCMV-BD"
                     /mol_type="other DNA"
     promoter        16..568
                     /label="CMV_immearly_promoter"
     misc_feature    71..358
                     /label="CAG_enhancer"
     misc_feature    525..545
                     /label="CMV_fwd_primer"
     promoter        526..595
                     /label="CMV_promoter"
     promoter        620..639
                     /label="T3_promoter"
     misc_feature    675..1115
                     /label="Gal4_DBD"
     CDS             675..1238
                     /label="ORF frame 3"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     misc_feature    1079..1097
                     /label="Gal4_N_term_primer"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     promoter        complement(1232..1250)
                     /label="T7_promoter"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     misc_feature    1307..1366
                     /label="SV40_3_splice"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     terminator      1417..1645
                     /label="SV40_PA_terminator"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     misc_feature    1615..1634
                     /label="EBV_rev_primer"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     rep_origin      complement(1784..2090)
                     /label="f1_origin"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     promoter        2169..2197
                     /label="AmpR_promoter"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     misc_feature    complement(2263..2283)
                     /label="pBABE_3_primer"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     misc_feature    complement(2269..2484)
                     /label="SV40_enhancer"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     promoter        2281..2549
                     /label="SV40_promoter"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     rep_origin      2448..2525
                     /label="SV40_origin"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     misc_feature    2510..2529
                     /label="SV40pro_F_primer"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                     KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                     NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPELGSASPGEFGS
                     LRPRRPLDCSSTSRGGPVPGKCTQFAL*"
     CDS             2632..3426
                     /label="ORF frame 1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            2635..3423
                     /label="NeoR/KanR (variant)"
                     /gene="NeoR/KanR (variant)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(2941..3477)
                     /label="ORF frame 3"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      3601..3870
                     /label="TK_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     rep_origin      4018..4637
                     /label="pBR322_origin"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
ORIGIN
    1 ATGCATTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA
   61 GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG
  121 CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG
  181 ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA
  241 TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC
  301 CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC
  361 TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC
  421 ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA
  481 TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG
  541 GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA
  601 GCGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GAGCTGATCT
  661 AAGCCTCCTG AAAGATGAAG CTACTGTCTT CTATCGAACA AGCATGCGAT ATTTGCCGAC
  721 TTAAAAAGCT CAAGTGCTCC AAAGAAAAAC CGAAGTGCGC CAAGTGTCTG AAGAACAACT
  781 GGGAGTGTCG CTACTCTCCC AAAACCAAAA GGTCTCCGCT GACTAGGGCA CATCTGACAG
  841 AAGTGGAATC AAGGCTAGAA AGACTGGAAC AGCTATTTCT ACTGATTTTT CCTCGAGAAG
  901 ACCTTGACAT GATTTTGAAA ATGGATTCTT TACAGGATAT AAAAGCATTG TTAACAGGAT
  961 TATTTGTACA AGATAATGTG AATAAAGATG CCGTCACAGA TAGATTGGCT TCAGTGGAGA
 1021 CTGATATGCC TCTAACATTG AGACAGCATA GAATAAGTGC GACATCATCA TCGGAAGAGA
 1081 GTAGTAACAA AGGTCAAAGA CAGTTGACTG TATCGCCGGA ATTGGGATCC GCTAGCCCGG
 1141 GCGAATTCGG AAGCTTGCGG CCGCGTCGAC CTCTAGACTG CAGCTCGACC TCGAGGGGGG
 1201 GCCCGGTACC AGGTAAGTGT ACCCAATTCG CCCTATAGTG AGTCGTATTA CAATTCACTC
 1261 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGAGATCCAA TTTTTAAGTG
 1321 TATAATGTGT TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCAC AGTCCCAAGG
 1381 CTCATTTCAG GCCCCTCAGT CCTCACAGTC TGTTCATGAT CATAATCAGC CATACCACAT
 1441 TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA
 1501 AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA
 1561 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT
 1621 TGTCCAAACT CATCAATGTA TCTTAACGCG TAAATTGTAA GCGTTAATAT TTTGTTAAAA
 1681 TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA
 1741 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC
 1801 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG
 1861 GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT
 1921 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG
 1981 GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA
 2041 AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG
 2101 GGCGCGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC
 2161 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA
 2221 TATTGAAAAA GGAAGAATCC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG
 2281 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT
 2341 AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA
 2401 TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA
 2461 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG
 2521 AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG
 2581 GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA
 2641 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC
 2701 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG
 2761 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG
 2821 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT
 2881 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG
 2941 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG
 3001 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA
 3061 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAACATCAG
 3121 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT
 3181 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT
 3241 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG
 3301 GCTACCCGTG ATATTGCTGA AGAACTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT
 3361 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC
 3421 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC
 3481 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG
 3541 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCTA
 3601 GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG
 3661 CAATAAAAAG ACAGAATAAA ACGCACGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC
 3721 GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC
 3781 GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG
 3841 CCAACGTCGG GGCGGCAGGC CCTGCCATAG CCTCAGGTTA CTCATATATA CTTTAGATTG
 3901 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA
 3961 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
 4021 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
 4081 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
 4141 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT
 4201 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
 4261 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
 4321 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
 4381 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
 4441 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
 4501 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
 4561 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
 4621 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
 4681 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC
//

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