GenBank: DQ188840LOCUS pGL4.20[luc2/Puro] 5404 bp DNA SYNDEFINITION pGL4.20[luc2/Puro]ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..5404 /organism="pGL4.20[luc2/Puro]" /mol_type="other DNA" CDS 100..1752 /label="ORF frame 1" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*" terminator complement(1793..1912) /label="SV40_PA_terminator" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*" misc_feature complement(1807..1826) /label="EBV_rev_primer" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*" misc_feature complement(2134..2154) /label="pBABE_3_primer" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*" misc_feature complement(2140..2355) /label="SV40_enhancer" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*" promoter 2152..2420 /label="SV40_promoter" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*" rep_origin 2319..2396 /label="SV40_origin" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*" misc_feature 2381..2400 /label="SV40pro_F_primer" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*" CDS complement(2454..3116) /label="ORF frame 3" /translation="MTGSRSLGARFSCHTPGPRSLGHLDIGGHGEAQALVEWKVSWCA CFEKGGHTGAFGGLYAGQHDGAAQALALVVGAHSYSGQKPGRLLGTMGGKQALHLLLG GQAAAAQLGHSGADLGEHGAGLNALGGSPHGHGRAVVGYPHLADVQPDAGQEQLLQLC HTLDVPVGVHGVPGSGVVGESGGQSADGAGHIIASGQAHGRLVLGHGGASVRRIELFA KA*" CDS 2499..3098 /label="ORF frame 3" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" terminator 3123..3171 /label="synth_PA_terminator" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" rep_origin complement(3483..4102) /label="pBR322_origin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" CDS complement(4286..5146) /label="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" terminator 5251..5404 /label="up_PA_terminator" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" terminator 5251..5299 /label="synth_PA_terminator" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 5251..5404 /label="TB" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 5353..5372 /label="RVprimer3_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN 1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC 61 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT 121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC 181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC 241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG 301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG 361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC 421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC 481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA 541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC 601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC 661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC 721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC 781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC 841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC 901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG 961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC 1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG 1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC 1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC 1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC 1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT 1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC 1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC 1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC 1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG 1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA 1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC 1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG 1741 ATCGCCGTGT AATAATTCTA GAGTCGGGGC GGCCGGCCGC TTCGAGCAGA CATGATAAGA 1801 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT 1861 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC 1921 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA 1981 AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGATAAGG ATCCGTTTGC GTATTGGGCG 2041 CTCTTCCGCT GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT 2101 TAGCTACCTT CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA 2161 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 2221 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 2281 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA 2341 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 2401 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT 2461 TTTGCAAAAA GCTCGATTCT TCTGACACTA GCGCCACCAT GACCGAGTAC AAGCCTACCG 2521 TGCGCCTGGC CACTCGCGAT GATGTGCCCC GCGCCGTCCG CACTCTGGCC GCCGCTTTCG 2581 CCGACTACCC CGCTACCCGG CACACCGTGG ACCCCGACCG GCACATCGAG CGTGTGACAG 2641 AGTTGCAGGA GCTGTTCCTG ACCCGCGTCG GGCTGGACAT CGGCAAGGTG TGGGTAGCCG 2701 ACGACGGCGC GGCCGTGGCC GTGTGGACTA CCCCCGAGAG CGTTGAGGCC GGCGCCGTGT 2761 TCGCCGAGAT CGGCCCCCGA ATGGCCGAGC TGAGCGGCAG CCGCCTGGCC GCCCAGCAGC 2821 AAATGGAGGG CCTGCTTGCC CCCCATCGTC CCAAGGAGCC TGCCTGGTTT CTGGCCACTG 2881 TAGGAGTGAG CCCCGACCAC CAGGGCAAGG GCTTGGGCAG CGCCGTCGTG TTGCCCGGCG 2941 TAGAGGCCGC CGAACGCGCC GGTGTGCCCG CCTTTCTCGA AACAAGCGCA CCAAGAAACC 3001 TTCCATTCTA CGAGCGCCTG GGCTTCACCG TGACCGCCGA TGTCGAGGTG CCCGAGGGAC 3061 CTAGGACCTG GTGTATGACA CGAAAACCTG GCGCCTAATG ATCTAGAACC GGTCATGGCC 3121 GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GTTCGAACTA 3181 GATGCTGTCG ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC