| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pcDNA3.2/V5-DEST |
| 质粒类型: | Gateway载体;哺乳动物表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | Gateway |
| 载体大小: | 7711bp |
| 5' 测序引物及序列: | T7 Forward:TAATACGACTCACTATAGGG |
| 3' 测序引物及序列: | V5 Reverse: 5′-ACCGAGGAGAGGGTTAGGGAT-3′ |
| 载体标签: | V5 Epitope Tag |
| 载体抗性: | 氨苄青霉素、氯霉素(仅空载体) |
| 筛选标记: | Neomycin |
| 克隆菌株: | DB3.1(空载体复制);OmniMAX2-T1 、DH5α 用于重组载体复制 |
| 表达细胞(系): | 常规细胞系如293、Hela等 |
| 备注: | pcDNA3.2/V5-DEST载体是Gateway载体,不能单独使用; 需要配合供体载体、入门载体。 |
| 产品目录号: | 12489-019 |
| 稳定性: | 瞬表达 或 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 技术资料
- 保存条件:
-20℃低温保存
- 保质期:
三年
- 英文名:
pcDNA3.2/V5-DEST
- 库存:
20
- 供应商:
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息





载体简介
The pcDNA vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features:
Cytomegalovirus (CMV) promoter for high-level expression
attR sites for Gateway cloning, enabling recombination with attL-flanked fragments
C-terminal V5 tag for easy detection
Neomycin resistance gene for stable selection
Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Gateway Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate ORF Clone. The following table lists a variety of available destination vectors.
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pcDNA3.2/V5-DEST载体
¥1800




