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- 文献和实验
- 技术资料
- 抗体名:
Mouse IgG2a Isotype Control APC Antibody 抗体
- 抗体英文名:
Mouse IgG2a Isotype Control APC Antibody
- 浓度:
0.1 mg/ml
- 应用范围:
FC
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb222804
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
APC
- 克隆性:
Monoclonal
- 保存条件:
详询
- 亚型:
Mouse IgG2a kappa
- 免疫原:
详询
- 规格:
0.1 mg
产品子类型: Secondary Antibody
描述: Mouse IgG2a Isotype Control (APC)
克隆号: MOPC-173
应用说明: Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
免疫原: The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
防腐剂: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
纯化方法: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
特异性: This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Entrez 基因 ID: 35
保存条件: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
备注: For research use only.

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, isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1 Isotype-Dependent Properties of Monoclonal
【翻译】Development trends for monoclonal antibody cancer therapeutics
as IgG2a, four mAbs were IgG3, four were IgG1, and one mAb was known only to be IgG. No correlation was observed between the isotype of the naked murine mAbs and the available information on the primary reason for termination of development (for example
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