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- 文献和实验
- 技术资料
- 保存条件：
详询说明书
- 库存：
10
- 供应商：
上海乔羽生物
- 规格：
200 U
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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
RNA Purification Protocol for 100-200 mg of Animal Tissue
at 3,000-5,000 x g for 15 minutes at room temperature. 9. Pour of the supernatant and drain the tube briefly on a clean absorbent paper. Add 6 ml of 70% ethanol and invert the tube few times to wash the RNA pellet. 10. Centrifuge at 3,000-5,000
BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets
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