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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 库存:
现货
- 供应商:
联硕生物
- 规格:
1瓶
应用
ProClin™ preservatives are immediately microstatic upon contact with microbial organisms as a result of their ability to quickly penetrate cell membranes and inhibit specific enzymes crucial to cellular respiration. Multiple specific sites of ProClin™ toxicity prevent microorganisms from developing high levels of resistance, making ProClin™ highly efficient even over extended storage periods of your IVD components.
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文献和实验1.3.1.偶联时间:2h 1.3.2.封闭时间:1h 1.4. 封闭要求:封闭时间不宜小于1h。 2. 偶联流程 2.1. 配制偶联实验所用的缓冲液,分别有以下几种: 2.1.1 偶联液:100mM MES pH4.8 2.1.2 封闭液:3M Ethanolamine,1%BSA,0.5%T-20,pH8.0 2.1.3 保存液:0.05%proclin-300的PBS缓冲液或者根据自己的需求配制其他缓冲液。 2.2. 先将磁珠用旋涡
Obtaining Embryos: hCG Injection
for males; 200 u hCG for females). Overnight they will lay eggs which can be collected first thing in the morning. However, for IVF, we prime females with 10 u hCG the night before we would like to obtain eggs. At the same time, we boost the males
RNase-free DNaseI (10 U/µl), 5 µl 0.1 M Tris-Cl pH 8.3, 5 µl 0.5 M KCl, 5 µl 15 mM MgCl 2 phenol extract once precipitate with 5 µl NaAcetate and 200 µl 100 % Ethanol incubate at least for 30 min at -80 °C spin, wash
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