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文献和实验Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
#2 and #3). 2. Add an equal volume of Phenol:Chloroform, mix well by inversion, and centrifuge in a microcentrifuge at full speed for 3 min to separate the phases. Save the aqueous phase (upper layer). 3. To the aqueous phase, add an equal
from the reservoir). 5) Sterilize the above parts by steam autoclaving at 121 °C for 60 min. Additionally autoclave one complete flow chamber, 3 x 2" segments of soft tubing, 1 male and 1 female 1/8" luer adaptor, 4 x ½" and 6 x 5/16" 8-32 flat head
Two dimensional peptide mapping
from the protease adsorbing onto the filter. 3. Aspirate the liquid. Wash the membrane extensively with H2O (5 X 1ml). Then wash with freshly-made 0.05 M NH4HCO3 once or twice. 4. Digestion: Add 150 -200 microliter fresh 0.05 M NH4HCO3
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