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文献和实验Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
5, was used for transformation by vacuum infiltration. Construction of pGKB5 A new plant transformation vector was designed, that could be used for T-DNA tagging in Arabidopsis. The T-DNA region of this plasmid is flanked by fragments containing
In Planta Transformation of Arabidopsis
(or equivalent) Cover for plants, transparent Dessicator with vacuum pump and glass dish (optional, for Steps 13-16) Incubator, preset to 28°C Spray bottle (optional, for Steps 17-19) Standard equipment for growing Arabidopsis (see Step
Purification of Demethylated Sphingomyelin 纯化脱甲基鞘磷脂
:1) Dry on rotovapor system with vacuum pump. Dry as completely as possible.a) Will be left with with an oil that smells very bad (because of the DMSO)2) Resuspend in ~2 ml chloroform.III. Interphase:1) Dry as much as possible on rotovapor
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