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上海希言科学仪器有限公司
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文献和实验and place into newly made EtBr solution and rock for approximately 10 min. 2. Rinse in H 2 O and take photo of gel. 3. UV irradiate gel for 1.5 min on transilluminator. 4. Slowly rock gel for 30 min in Denaturation Solution.
to transfer overnight (setup should have a pyramid shape) Random-Primed Labeling of DNA Fragment: For probe synthesis, you can use a commercially available random-primed labeling kit using α-32P-dCTP (i.e., Roche) or DIG-labeled probe (Roche
and lock box (from various vendors) Mini-centrifuge (VWR, 20668-212) Thermolyne Dri-Bath, Type 17600 (VWR, 35756-006) Platform Shaker, New Brunswick Scientific (VWR, 40000-300) Electrophoresis Separation Systems (Owl) QIAquick Nucleotide Removal Kit (Qiagen
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