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文献和实验The new opportunities of modern assays of molecular biology can only be exploited fully if the results can be accurately correlated to the tissue phenotype under investigation. This is a general problem of non-in situ techniques
Primer Design(Manual and Automated Primer Design for SEQ and PHY Gaps)
General Primer General Primer Design Guidelines Note the 5’-3’ direction of the contig. Locate primers 100 to 200 bp from the feature. Pick primer from ≥ 2x high quality sequence coverage
Marcantonio Lab Protocol Manual
sandwich back and forth. Pour the rinse off as waste. 3) Repeat the rinse as above. 4) Add the following reagents to the stacking gel solution: TEMED 40 ul 10% Ammonium Persulfate 200 ul Following addition of the APS, stir the solution
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