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文献和实验RAMPAGE: Promoter Activity Profiling by Paired‐End Sequencing of 5′‐Complete cDNAs
) Molecular‐biology grade water (Sigma‐Aldrich, cat. no. 95284‐100ML) Agencourt RNAClean XP kit (Beckman Coulter, cat. no. A63987
A Proteomics Approach to Identify Redox-Sensitive Proteins
, respectively. Every second, a TOF MS precursor ion spectrum is accumulated, followed by three product ion spectra, each for 3 s. The switching from TOF MS to MS/MS is triggered by the mass range of peptides (m/z 300–2,000), precursor charge state (2–4), and ion
System (biotinylated probe): DNA - 1µg dNTPs 10X Mix - 5µl Enzyme 10X Mix - 5µl ddH2 O (from the kit) - to 50µl final Mix well and centrifuge briefly; Incubate 1-2 hours at 16°C. Before stopping the reaction, load 1/10 (5µ
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