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文献和实验Planar Lipid Bilayer Experiment平面脂双层实验
down completely in a vacuum until can?t smell the CHCl3, resuspend the white powder in 0.133 ul decane with 5 ul of n-butanol to yield 1.5% solution of cholesterol, vortex, can store this in fridge, sealed to prevent moisture getting in. Also, don?t open vial
OLIGO PURIFICATION on acrylamide gels
and place into a 10ml tube with 1ml TE. Allow to elute overnight with agitation (Optional: break up gel slice by passing through a stop cock from one syringe to another before eluting over night). 7. Filter the eluate through a pre-wetted acrodisk. Collect
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
medium, then plate all onto a 10cm dia petri dish containing NeoPMEFs and 10ml medium. Label plate 2. 5. Set up following control plates. a) Take 0.167 ml (2 x 106 cells) original cell suspension _ 10cm dia petri dish containing NeoPMEF's and 10ml
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