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文献和实验Disulfide Conjugation of Peptides to Oligonucleotides and Their Analogs
. However, severe difficulties have been encountered in carrying out conjugations between highly cationic peptides and negatively charged oligonucleotides because of aggregation and precipitation. Presented here are reliable protocols for disulfide conjugation
1.5 (bromoacetylation) and 1.6 (displacement). 8) After the final displacement is done, rinse with 2 mL of DMF (repeat 4x), then 2 mL of dichloromethane (repeat 3x). Cap and store the reaction vessel until cleavage. 9) Pause in synthesis
Synthesis of Oligodeoxynucleotides with 5′‐Caps Binding RNA Targets
acid cap), 1C95 (tryptophan cap), 1KSE (oxolinic acid cap), and 1X6W (trimethoxystilbene cap). The DNA is shown in gray and the stacking moiety of the caps are shown as orange line drawings with smaller line width
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