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文献和实验Dry Transfer Trans-Bot SD Assembly1. Prepare the transfer buffer.2. Following electrophoresis, equilibrate the gels in transfer buffer. Equilibration facilitates the removal of electrophoresis buffer salts and detergents. The length of time
cover and the cathode assembly. Discard the filter paper (and dialysis membrane, if used). The transfer efficiency can be monitored by staining the gel with Coomassie blue R-250 protein stain or with Bio-Rad’s Silver Stain Kit. Alternatively
reporter plasmids (TCF reporter plasmid kit; Upstate) is needed. B Pharmacological inhibition ofGSK-3 activity. MiaPaCa2 pancreatic cancer cells do not express E-cadherin and there is free (free from binding to Ecadherin)cytosolic β-catenin pool
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