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文献和实验GST Fusion Protein Preparation
than 1.0.Prior to induction,remove 100μl cells and add 100μl SDS-PAGE sample buffer.Boil briefly and load 10μl for SDS-PAGE.Sharper bands can be obtained if you pass through syringe several times to fragment DNA.Collect hourly samples to determine optimal induction time.Pour
/240 μl 4. Seal the reaction tubes carefully with the strip caps, and centrifuge briefly at 2500 rpm. Place the tube/retainer set in the 9600 Cycler, abort the soak file program, and run program #11. This program will cycle the sequencing reactions
Electrophoretic Mobility Shift Assay(EMSA)
, bottom and side with binder clips• Lie on a slight angle (can use microfuge tube rack) Native PAGE Preparation:Add in this order63.25ml dH2O3.75ml 10x TBE7.5ml 40% 37:5:1 acrylamide0.5ml 10% APS50μl TEMED0.5x TBE: 50ml 10x TBE,950ml dH2O Gel Preparation Before Sample
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