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文献和实验Kingfisher Flex 96 Plant DNA High Pure Protocol
for pipette 7. Ice Bucket 8. 1.5 or 2.0 mL microcentrifuge tubes, sealed deep-well plate or capped microtube rack for sample disruption 9. Kingfisher 96 or Kingfisher Flex 96 with Deep Well Magnet 10. 4 Deep Well Kingfisher 96 Plates
E-Z 96 X-press Plasmid Spin Protocol
with swinging-bucket rotor capable of 3,000 x g 2. Vacuum Manifold (Cat# Vac-03) 3. Vortex 实验步骤 1. Process the cell lysate by following step 1-6 from vacuum protocol. 2. Place a E-Z 96 X-press plate on top of a 96 deep
E-Z 96 X-press Plasmid Vacuum Protocol
selective antibiotic. Incubate 14-16 hours at 37℃ 600 with vigorous shaking until an OD of 2.0-4.0 is achieved. 2. Pellet 1-2 mL bacterial culture in a 2.0 ml 96-well deep-well plate by centrifugation at 3000 x g for 15 min at room temperature.
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