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文献和实验will give the desired celldensity to perform the assay. Most of the following manipulationscan be performed with 12-well multichannel pipetters. 2. Prepare samples to be tested in a separate 96-well plate: To100 μL of cell culture media, add 1–2 μL of the Wnt
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Approximately 100 μl of radiolabeled DNA 15 μl of 10X Restriction Enzyme Buffer 15 μl of 10 mM DTT 1.5 μl of 20 mg/ml BSA 20 Units of Restriction Enzyme ddH2 O to give an approximate final volume of 150 μl
Lipoprotein Isolation - First week
100 ml beaker and add 25 µl of 200 mM PMSF Caution! Highly poisonous! picture ). Make sure no insect saliva drips into the beaker (have Kleenex ready to wipe off saliva). 3. Gently inject a few hundred microlitres of PBS
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