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文献和实验Polyacrylamide Gel Electrophoresis of Oligonucleotides
1. Pour and polymerize a 20% polyacrylamide gel, no Urea. 2. Remove clamps. Rinse with water. Remove comb. Rinse top of gel well. 3. Insert comb teeth down until teeth just touch the gel. Mount the plates in apparatus. Fill chambers
Working Procedures in Microwave Histology
Hematoxylin for 1 minute Blue rinse in tap water; dehydrate, clear and mount Note: This procedure was adapted from "The Microwave Cookbook for Microscopists", Boon and Kok, Coulomb Press Leiden, 1992 PAS Procedure: PAS done at room
Use of Channelrhodopsin for Activation of CNS Neurons
) Blue light laser with fiber coupler (e.g., 10 mW, 473‐nm diode‐pumped solid‐state continuous wave laser system with FC/PC multimode fiber coupler; OEM Laser Systems)
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