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文献和实验screws, 1 pair of tweezers, one 75 x 25 x 1 mm glass slide (or other desired cell surface material), and 2 surgical towels. 6) Place sterile towels into laminar flow hood. Place the flow circuit, flow chamber, 4 x 4-way stopcocks, 1 x 1-way
Purification of recombinant sBRF M166L
J.Movius,K,Coachman,and S.Hahn (Hahn Lab) last modified 10/9/00 Induction of BRF in bacteria and purification on Ni-NTA agarose Transform BRF plasmid into strain BL21 DE3 (pLysS)or JM25 (DE3 containing the Arg tRNA over expression plasmid
A Method for Micro-Scale Isolation and Purificatio
caps (#8142) Protocol: Phases: Organic : 2 volumes DIPE/1-butanol, 60:40 v/v Aqueous : 1 volume saline solution Aqueous phases are as follows: Cell pellets : for cells, use 0.l% NaCl: 10 ml saline
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