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文献和实验from direct light, use within 5 min. Second stain: Double indirect immunohistochemistry (e.g rabbit anti antigen X, goat anti-rabbit AP, rabbit anti-goat AP) A second blocking step is omitted, because blocking is due to the first stain.
in the same species. However better results are obtained if two different species are used. Careful check possible crossreactivty of your secondary antibodies by staining in simple IHC the relevant heterologous primary antibodies. E.g. many goat anti mouse
Transformation of E. coli by Electroporation
Chamber. 7. Set the resistance on the Voltage Booster to 4 kΩ; set the Pulse Control unit to LOW and 330 FF; double check connections. 8. Charge the Pulse Control unit by setting the CHARGE ARM switch on the Pulse Control unit to CHARGE
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