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文献和实验hemocytometer. To get the final count in cells/ml, first divide the total count by 0.1 (chamber depth) then divide the result by the total surface area counted. For example if you counted 125 cells in each of the four large corner squares plus the middle, divide
Sample preparation (preparative gels)
cell debris and blood and then immersed into an iced PBS solution containing 3 mM EDTA, 50 µg/ml leupeptine, 0.2 mM PMSF and 0.8 mM benzamidine. Crypts were scraped away from the basal membrane with a scalpel. The tissue was then gently pressed
by centrifugation x 5 min at 600g and resuspend in 5 ml of EPC medium. 6. Cell seeding of slide prior to flow chamber assembly 1) A slide made out of glass, titanium, or other material can be used, and the slide surface can be modified by spin
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