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文献和实验any embryos. Shake once again. Now all of the embryos should settle to the bottom. Remove liquid and wash 2-3x with methanol. -larvae at the bottom of the scintillation vial are generally wild type. They can also be drawn up in the aqueous solution in a P
and wash 2-3x with methanol. -larvae at the bottom of the scintillation vial are generally wild type. They can also be drawn up in the aqueous solution in a P-1000 and transferred to an eppendorf tube. Remove the majority of liquid and add around 0.5 ml
so that a 75 x 25 x 1 mm microscope glass slide will sit flush in the chamber. 7) Create a recess for an O-ring around the underside of the top and bottom plates such that they are spaced apart by 0.02". Insert the rubber O-rings. 8)
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