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文献和实验from the reservoir). 5) Sterilize the above parts by steam autoclaving at 121 °C for 60 min. Additionally autoclave one complete flow chamber, 3 x 2" segments of soft tubing, 1 male and 1 female 1/8" luer adaptor, 4 x ½" and 6 x 5/16" 8-32 flat head
. Take the precipitate using a sterile transfer pipet (IWAKI 7801-002) (Do not suck! Wind the precipitate around the pipet.) or by centrifuging Dissolve the precipitate in (2/5 x X) ml TE containing 1 M NaCl and RNase A (1 to 10 ug/ml). Incubate at 55oC for 1 hr
; pH = 8.0 (store at 4o C). Conc. Stock %(w/w) 50 ml 100 ml 150 ml 250 ml EDTA 0.5 M 372.3g/M 16.98 9.31g 18.62g 27.92g 46.55g NaOH ~0.5 M 40g/M 1.82 1.014g 2.028g 3.042g
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