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文献和实验from the reservoir). 5) Sterilize the above parts by steam autoclaving at 121 °C for 60 min. Additionally autoclave one complete flow chamber, 3 x 2" segments of soft tubing, 1 male and 1 female 1/8" luer adaptor, 4 x ½" and 6 x 5/16" 8-32 flat head
Array CGH of Microdissected Fresh or Formalin Fixed DNA
into chamber taking care to keep the slides level, and to avoid disrupting the rubber cement. Seal the chamber with a layer of rubber cement, then a layer of parafilm. Keep the bottom of chamber as flat as possible, do not fold the parafilm
Spotted Microarray Hybridization and Scan
to pellet any particulate material Place the Microarray(s) in a hybridization chamber (TeleChem or GeneMachines)(To prevent arrays from drying, seal array in hybridization chamber with Whatmann paper moistened with 100 ml of H2 O.)
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