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上海希言科学仪器有限公司
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SEAL KIT,PISTON,10ML ( 120798 )
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文献和实验相关实验
immediately or store filter, either a) rinse in TBSt, place in seal-bag and store at 4°C, or b) place in Blocking Solution in seal-bag and store at -20°C. Note: Be aware of what type of membrane you are using as it could affect how you want to store. V
In Situ HYBRIDIZATION TO TISSUE SECTIONS
ml glass tube -Spot 10ml on a GF/C filter disk -To the remaining 90ml, add 5ml cold 10% TCA; incubate 10' on ice -Filter onto GF/C, wash with 10% TCA, wash with 95% EtOH and dry -20-50% incorporation is good and corresponds to a S.A. ‰ 1-2 x 108
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SEAL KIT,PISTON,10ML ( 120798 )
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