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文献和实验James Hardwick CNBr Cleavage Procedure
tube containing 200 microliters of 100mg/ml CNBr in 70% formic acid. CNBr stocks are 200 milligrams/ml in 70% formic acid and are stored at -70°C. 70% formic acid is made from 98-100% formic acid and MQH2O. Optional: If you want to know
to precipitate as long as desired (up to overnight)。 To proceed, spin sample in a 4 !C microfuge at max speed for 30 minutes to 1 hour. 12. Aspirate off supernatant and wash pellet with 250 L 70% EtOH and spin down 5 . polyA RNA Isolation 13. Aspirate
Sequencing-Template Preparation, DS测序模板准备【Upstate Medical University】
)and take the volume to 24μl with H2O. 2.Denature at 85℃ for 5 min.and plunge into a ice water bath.Add 3μl cold ammonium acetate (2M pH 4.5)and 70μl 100% EtOH.Store at -20℃ for 1-2 hrs. 3.Spin down DNA 10 min at high speed.Wash pellet with 70% EtOH,repeat a short spin
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