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文献和实验UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
temperature 10o to 15o below the vessel temperature. This will circulate water through the cooling system. After about a minute, increase the temperature to the desired growth temperature. 4. Set sterilization time (60-90 min usually). Set desired growth
Replication timing by density transfer
, and microtitre plates for the denaturation/neutralizing: Mix 42 microliters of DNA (in CsCl) with 28 microliters of 1 N NaOH (=> 0.4 N NaOH final conc.). Seal the plate with an adhesive sheet and incubate at 65o C for 1 hr. Cool to room temperature
Determining the Direction of Replication Fork Movement
onto the surface of the gel, 10 to 20 microliters of enzyme. Seal the reservoir with parafilm and incubate at 37o . (Alternatively, set the reservoir in a glass blaking dish with water in the bottom and cover with saran wrap.) Digest 4 hours (or more).
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