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文献和实验96 Well Mating Protocol platewise
on the XGAL plate 2. Streak recipient plasmid (pMSCV) on LB Spec50 CB100 0.2% glucose OR YEGlu spec50 cb100) Grow at 30oC overnight Protocol 1. Inoculate 5 ml single colony culture of recipient, and a 1:100 donor culture in 500 ul/well 96 well culture box
96 Well Mating Protocol platewise
+Spec50+CB100+0.2% glucose OR YEGlu+spec50+cb100) Grow at 30oC overnight Protocol1. Inoculate 5 ml single colony culture of recipient, and a 1:100 donor culture in 500 ul/well 96 well culture box. Grow recipient at 30oC and donor at 37℃, shaking
96-well Plate Dual Luciferase Assay--96孔板荧光素酶检测
Reagents: Plasmids: can be found in my plasmid(1) box in the ?20oC freezer. 985 : FR-tk-luciferase 2517 : Renilla-tk-luciferase 2145 : GFP Lipfectamine and lipofectamine plus reagents: in the upper shelf of the medium freezer in tissue
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