Eppendorf Reference® 2 单道可调量程

Chromatin immunoprecipitation on native chromatin from cells and tissues

fraction P, in 50µl of dialysis buffer and store at 4°C. Quality Control of Chromatin Take the Optical Density (OD) of each fraction at 260nm; In separate 1.5mL Eppendorf tubes, put 0.5µg of each fraction (S1, S2, and P);

A/B/C Random Amplification Protocol

DNA. Round B PCR   30 µL  Round A DNA product   20 µL 10X Thermo Pol PCR Buffer     2 µL 25 mM dNTP     4 µL  100 µM Primer B Cy5 (sample) or Cy3 (reference DNA)     2 µL  Ampli Taq  0.1 µL Pfu Turbo 142 µL dH 2 O    

Sample Preparation for Microinjection

Buffers, e.g., PB’s, are not recommended as this often leads to blockages of the capillaries. DNA concentrations of 20 µg/ml to 200 µg/ml for plasmid DNA injection has been recommended [1] . Storage Store at �20°C in small aliquots of 5�10 µl