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文献和实验High Throughput Isolation Of PCR Products Using ChargeSwitch® PCR Clean-Up
. For more information, see http://www.invitrogen.com or call Technical Service. Follow the protocol below to purify PCR products. The volumes given are on a per sample basis. 1. To ~50 µl PCR samples in 96-well plates, add 10 µl ChargeSwitch® Magnetic Beads.
1 µl of air. 6. Load 2 µl of Loading Buffer from the reservoir. 7. Place tips in clean wells of disposable mixing tray and allow pipette to mix the sample and loading dye. 8. Place the pipette tip in a 50 well row so that the tip
Gene Knockout Techniques(1) The Knockout Process
time of harvesting depends on the worm plates ? harvest when the wells have just starved. Prepare 12 96-well 200 ul PCR plates, labeled A through L with rows numbered 1-8 and containing 50 ul Proteinase K buffer in each well. Harvest each worm
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