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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD86
- 亚型:
Mouse IgG1
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
APC
- 适应物种:
Hu
- 宿主:
Mouse
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:BU63
- 抗体英文名:
CD86 Antibody-APC;流式抗体
- 抗体名:
CD86 Antibody-APC;流式抗体
- 规格:
2X10^6
克隆类型 :Mouse Monoclonal Antibody;Clone number:BU63
货 号 :HT1611011
规 格 :25/100 tests ;
亚 型 :
Mouse IgG1
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
适用物种 :
Hu
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used from 20 µL to 5 µL per 100 µL of peripheral blood.
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
APC(其他荧光标记请咨询我们)
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文献和实验CD86 分子 CD86 常用单克隆抗体或代号: FUN- 1,BU63,GR65;(B7- 2) 主要表达细胞: Ba,M [B] 分子质量(kDa)和结构: gp80(IgSF) 功 能: CD28、CTLA- 4配体,提供T细胞共刺激信号 CD86 Summary: Costimulates IL-2 production and T cell proliferation
Incorporation of Immunomodulators into Plasmid DNA Vaccines
(CD80, CD86, and CD40-ligand) may provide maturation signals to antigen-presenting cells (APC) to enhance their ability to present vaccine antigens, and result in an increased adaptive immune response (3 ,4 ). More recently, cytokines and chemokines
,普遍认为(也可能是我自己认为),第6天或第8天收不成熟DC会比较多,看实验需要,建议想要不成熟DC的朋友请在这两天收网)。 我一般做到第5步就上流式,也就是6到8天的BMDC, CD11c表达波动在50-80%,偶尔做CD86,成熟标志吧?15%左右。具体流式结果和光镜形态照片过几天(或几周)发上来大家一起探讨。MHC2我没有做(技术员说实验室流式机器做APC的有关部件有故障,我的MHC2抗体正好带APC)。还发现用PE标记和FITC标记的CD11c流式抗体检测CD11c表达发现阳性
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