- ￥3000 - 3999
- Abbiotec
- 美国
- 250870
- 2025年07月04日
- E, WB, IHC
- Rabbit
- H, M, R
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SPR.
- 亚型：
Rabbit Ig
- 形态：
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 25% glycerol, 0.1 mg/ml BSA with 0.09% sodium azide. Antibody was purified by Protein-A and immunogen affinity chromatography.
- 保存条件：
Store at -20°C.
- 克隆性：
多克隆抗体
- 标记物：
见下方详细说明
- 适应物种：
H, M, R
- 宿主：
Rabbit
- 应用范围：
E, WB, IHC
- 浓度：
见详细说明信息
- 靶点：
Substance-P Receptor
- 抗体英文名：
Substance-P Receptor Antibody
- 抗体名：
Substance-P Receptor Antibody
- 规格：
0.1 mg
Substance-P Receptor Antibody产品详细介绍：
| 产品名称： | Substance-P Receptor Antibody |
| 说明书： | http://www.abbiotec.com/antibodies/substance-p-receptor-antibody【参考图片】 |
| 货号： | 250870 |
| 规格： | 0.1 mg |
| 产品描述： | Substance-P receptor (SPR) is a receptor for the tachykinin neuropeptide substance P. SPR is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The affinity of SPR for tachykinins is substance P > substance K > neuromedin-K. |
| 克隆类型： | Rabbit Polyclonal Antibody |
| 亚型： | Rabbit Ig |
| 免疫原： | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SPR. |
| 适用物种： | H, M, R |
| 应用范围： | E, WB, IHC |
| 应用说明： | E: 1:500-1:1,000; WB: 1:200-1:500; IHC: 1:200-1:500 |
| 别名： | Substance-P receptor; SPR; NK-1 receptor; NK-1R; Tachykinin receptor 1; TACR1; NK1R; TAC1R |
| 形态： | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 25% glycerol, 0.1 mg/ml BSA with 0.09% sodium azide. Antibody was purified by Protein-A and immunogen affinity chromatography. |
| 保存条件： | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献： | Zhu H et al. 2010. Cornea.29(7): 794-2864. PMID# 20489575. |
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文献和实验antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000 - 1:20,000 dilution in 4% BSA in TBST. This should be titrated for optimal results. I incubate with primary Ab 3 hours - overnight at room temp with shaking.10. Remove the antibody solution
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
Determination of Substance Concentration by Competitive ELISA (zt)
the wells with 1% BSA/PBS (100 μl/well) for at least 1 hr at 37°C.4. Remove the 1% BSA/PBS by flicking the plate.5. Wash the wells three times with PBS/Tween6. Set up a competition assay between the antibody and the competing substance:Prepare 1:2 serial
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