- ￥1000
- Vazyme
- 南京
- TD601
- 2025年12月11日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
TruePrep Amplify Enzyme
- 库存：
充足
- 供应商：
诺唯赞生物
- 规格：
96 rxns
TruePrep Amplify Enzyme
文库扩增
用于DNA建库试剂盒TruePrep DNA Library Prep Kit V2 for Illumina中的扩增部分，包含文库扩增专用聚合酶，配以精心优化的反应缓冲，较大程度上保证了文库扩增的高效性和均一性，对不同起始量的模板均能实现高效稳定扩增，仅需1 ng起始DNA。可配套或补充TruePrep DNA Library Prep Kit V2 for Illumina (TD501/TD502/TD503)使用。
| 组分 | TD601 |
| TAE | 96 μl |
| 5 × TAB | 960 μl |
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文献和实验Two long PCR steps: First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA. First round of the nested PCR step of the limiting dilution step to amplify single templates
PCR PRIMER DESIGN AND REACTION OPTIMISATION
alignments; the mismatch positions were used as 4-base degeneracies for the primers (shown as stars; 5 in F and 4 in R), as shown above. Despite their degeneracy, the primers could be used to amplify a 250 bp sequence from viruses differing in sequence
vol 687 Chapter 2 采用DNA聚合酶融合技术进行长片段PCR扩增 （精华）
challenging,despite the availability of specialized long-range PCR enzymes.The majority of researchers use Taq blended with a small amountof proofreading enzyme (Taq blend) to amplify targets greater than10 kb in length (1). Such blends, however, exhibit
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