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- 详细信息
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- 文献和实验
- 技术资料
- 保存条件：
4℃
- 保质期：
1年
- 英文名：
ExoQuick exosome precipitation solution
- 库存：
100
- 供应商：
天津速徕生物科技有限公司
- 规格：
5ml/20ml
ExoQuick Overview
Need exosomes? SBI's ExoQuick products enable high-throughput, quantitative isolation of exosomes from low sample volumes (as little as 100µl serum). Compatible with any biofluid and a wide variety of downstream applications, ExoQuick is an effective and proven alternative to ultracentrifugation (refs 1-3).
ExoQuick is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.
Exosomes isolated with ExoQuick appear similar to exosomes isolated using ultracentrifugation in electron microscopy studies (refs 1,2), and are active in numerous functional assays (refs 2,3) Backed by a growing number of publications, ExoQuick is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models. ExoQuick exosome isolation methods are patented technologies (ref 4).
"We therefore pursued the Exoquick method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models."(ref 2).
The ExoQuick Family
ExoQuick for most biofluids
ExoQuick-TC for tissue culture and urine
ExoQuick-CG for pre-clinical in vivo applications
ExoQuick-LP to remove contaminating lipoprotein particles from plasma or serum before ExoQuick precipitation
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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
Introduction The efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replication) can be determined by trichloroacetic acid (TCA) precipitation. TCA
Protein Precipitation Protocols
TCA-DOC For precipitation of very low protein concentration 1) To one volume of protein solution, add 1/100 vol. of 2% DOC (Na deoxycholate, detergent). 2) Vortex and let sit for 30min at 4ºC. 3) Add 1/10 of Trichloroacetic acid (TCA) 100
). These amounts assume that the DNA is in TE only; if DNA is in a solution containing salt, adjust salt accordingly to achieve the correct final concentration. Mix well. Add 2 to 2.5 volumes of cold 100% ethanol (calculated after salt addition). Mix
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