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        Ethanol Precipitation of DNA

        互联网

        800
         
         

        Materials:

        3 M sodium acetate pH 5.2 or 5 M ammonium acetate

        DNA

        100% ethanol

        Measure the volume of the DNA sample.

        Adjust the salt concentration by adding 1/10 volume of sodium acetate, pH 5.2, (final concentration of 0.3 M) or an equal volume of 5 M ammonium acetate (final concentration of 2.0-2.5 M). These amounts assume that the DNA is in TE only; if DNA is in a solution containing salt, adjust salt accordingly to achieve the correct final concentration. Mix well.

        Add 2 to 2.5 volumes of cold 100% ethanol (calculated after salt addition). Mix well.

        Place on ice or at -20EC for > 20 minutes.

        Spin a maximum speed in a microfuge 10-15 min.

        Carefully decant supernatant.

        Add 1 ml 70% ethanol. Mix.

        Spin briefly.

        Carefully decant supernatant.

        Air dry or briefly vacuum dry pellet.

        Resuspend pellet in the appropriate volume of TE or water.

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