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250 µl
Amersham ECL Full-Range Rainbow Molecular Weight Markers
Full-Range Rainbow Molecular Weight Markers
- Monitor progress of protein electrophoresis and assess transfer efficiency and molecular weight of blotted proteins.without staining
- High intensity, prestained multicolored bands allow distinction of closely related proteins.
- Full-Range Rainbow Markers (Mr 12 000 to 225 000) use ten separate proteins with six different colors.
- High-Range Rainbow Markers (Mr 12 000 to 225 000) use eight separate proteins with six different colors.
- Low-Range Rainbow Markers (Mr 3 500 to 38 000) use seven separate proteins with five different colors.
- For use on SDS-PAGE.
Rainbow Molecular Weight Markers have been enhanced to enable faster and simpler identification of proteins on SDS-polyacrylamide gels. The ready-to-load markers provide sharper, more intense bands on gels and blots and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colors of the markers allow easier confirmation of transfer to blotting membranes and orientation.
Rainbow Molecular Weight Markers comprise a mixture of individually colored proteins that are combined to produce bands of equal color intensity. The approximate protein concentration is 1 to 2 mg/ml, depending on the product. Each 250 µl pack size is sufficient for use with 50 minigels (10 x 8 cm) or 25 large gels (20 x 20 cm).
Amersham ECL Full-Range Rainbow Molecular Weight Markers
| Complete Packsize | 250 µl |
| Order Information | Store at -15°C to -30°C. |
| Application | For industrial applications: Analytical reagent, research Use ten separate proteins with six different colors |
| Number of Tests | Quantities sufficient for use with 50 minigels (10 × 8 cm) or 25 large gels (20 × 20 cm). |
| Molecular Weight [Mr] Min. | Mr 12 000 |
| Molecular Weight [Mr] Max. | Mr 225 000 |
| Concentration | Approx. 2 mg/ml of each protein |
| Kit Contents | 1 vial, 250uL. Supplied ready to use in 305% glycerol and sample buffer containing mercaptoethanesulphonic acid (MESNA) as reducing agent. Sufficient for 25 standard SDS PAGE gel loadings or 50 mini-gel loadings. Full-range rainbow markers (Mr 12 000 to 225 000) use ten separate proteins with six different colors. |
| Physical State | Liquid |
| Color | Brownish-red |
| Solubility | Easily soluble in cold water and hot water |
| Stability | Stable |
| Shelf life | At least 3 months |
| Storage Conditions | -15 to -30°C |
| Wetted Part | No |
| For Use With | SDS-PAGE |
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文献和实验(pre-stained)好用，因为电泳过程中完全看不到，要和目标蛋白一起等到最后染色才“开蛊”，无法对实验起预示参照作用。完全属于“后知后觉”型的，当然还是比“不知不觉”不做对照的要好。 ① 宽分子量蛋白标准 如果从实验室总体考虑的，就选范围尽量宽，条带分布比较均匀的，这样你的蛋白无论在哪个区间都容易判断，避免正好落在一段空白区的危险。不过条带越多，每次上样量也就越费。拿到Marker后，如果买的是大包装，就应该考虑分装。多次反复冻融对于蛋白的危险，不用多说吧。另外要记得，宽
也有多种选择，选择joining valleys方式是比较好的。如果不扣背景，会导致大得离谱的误差。 注意条带峰的基线下面，其背景值与条带本身的面积差不多大了。对这张照片来说，如果不扣背景，测量的光密度值会增加一倍左右。 5. 设置marker 跑电泳时都要跑个marker的。这个marker实际上就是测量分子量的参照。软件可以利用这个参照来测量各条带的分子量。这个操作会罗索一点。需要作的是： a. 指定marker泳道。一般会默认第一
的，当然还是比“不知不觉”不做对照的要好。 ① 宽分子量蛋白标准 如果从实验室总体考虑的，就选范围尽量宽，条带分布比较均匀的，这样你的蛋白无论在哪个区间都容易判断，避免正好落在一段空白区的危险。不过条带越多，每次上样量也就越费。拿到Marker后，如果买的是大包装，就应该考虑分装。多次反复冻融对于蛋白的危险，不用多说吧。另外要记得，宽谱的Marker不一定每条都能看到的，特别是Mini Cell。如果侧重大蛋白，那小的可能就已经跑掉了，不是质量不好哦。 ② 高分子量
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