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- Cell Signaling Technology
- 5366
- USA
- 2025年09月12日
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- 抗体英文名：
Anti-rabbit IgG (H+L) (DyLight 680 Conjugate)__®
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 标记物：
DyLight 680
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
500 ul/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 500 ul | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
Secondary Antibodies
Anti-rabbit IgG (H+L) (DyLight® 680 Conjugate) #5366
Specificity / Sensitivity
Anti-rabbit IgG (H+L) (DyLight® 680 Conjugate) reacts with heavy and light chain of most rabbit immunoglobulins. No cross-reactivity to other serum proteins has been detected. This antibody may cross-react with immunoglobulins from other species.
Source / Purification
This antibody is prepared from goat antibodies and purified by immunoaffinity chromatography using antigen coupled to agarose beads.
Fluorescent Detection
Western blot analysis of Jurkat cell lysates (#9194) treated with either U0126 (MEK 1/2 inhibitor) #9903 or TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 detected with Anti-rabbit IgG (H+L) (DyLight® 680 Conjugate) (red) and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106 detected with Anti-mouse IgG (H+L) (DyLight® 800 Conjugate) #5257 (green). The array image pixel intensities obtained using a LI-COR® Biosciences Odyssey® Infrared Imaging System are shown in the upper panel while corresponding fluorescent western blots are shown in the lower panel.
In-Cell Western
In-Cell Western™ analysis of A549 cells exposed to varying concentrations of U0126 (MEK1/2 Inhibitor) #9903 for 3 hours, followed by TPA (Phorbol-12-Myristate-13-Acetate) #9905 stimulation for 30 minutes. With increasing concentrations of U0126, a significant decrease (~5 fold) in Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 signal as compared to the TPA-stimulated control was observed. When using phospho-Erk as a measurement, the IC50 of this compound was 1.9 μM. Data and images were generated on the LI-COR® Biosciences Odyssey® Infrared Imaging System using Anti-rabbit IgG (H+L) (DyLight® 680 Conjugate).
Description
Anti-rabbit IgG (H+L) was conjugated to DyLight® 680 fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 684 nm and peak fluorescence emission is 715 nm.
Directions for Use
The optimal dilution of the anti-species antibody should be determined by the user. However, the final dilutions below should yield acceptable results for the respective applications.
Fluorescent western blotting: 1:15000
In-Cell Western: 1:1000
Applications
This product has been optimized for use as a secondary antibody in fluorescent western blotting and In-Cell Western™.
Background
Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
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- 4413 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
- 4414 Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
- 4417 Anti-rat IgG (H+L), (Alexa Fluor® 555 Conjugate)
- 4418 Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate)
- 5151 Anti-rabbit IgG (H+L) (DyLight® 800 Conjugate)
- 5257 Anti-mouse IgG (H+L) (DyLight® 800 Conjugate)
- 5470 Anti-mouse IgG (H+L) (DyLight® 680 Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
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