- 询价
- Cell Signaling Technology
- 2284
- USA
- 2025年08月24日
- W, IHC-P, F
- Rabbit
- H,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Cathepsin D Antibody
- 抗原：
synthetic peptide corresponding to residues the heavy chain subunit of human Cathepsin D
- 应用范围：
W, IHC-P, F
- 宿主：
Rabbit
- 供应商：
CST
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 库存：
大量
- 适应物种：
H,Mk
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) F=Flow Cytometry
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P F | H Mk | Endogenous | 46, 43, 28 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Cathepsin D Antibody detects endogenous levels of preprocathepsin D, procathepsin D and the heavy chain subunit of mature cathepsin D. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues with the heavy chain subunit of human Cathepsin D. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from PANC1, SNB19 and MCF-7 cell lines using Cathepsin D Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Cathepsin D Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma using Cathepsin D Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human papillary carcinoma using Cathepsin D Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human serous adenocarcinoma using Cathepsin D Antibody. Flow Cytometry
Flow cytometric analysis of MCF-7 cells using Cathepsin D Antibody (blue) compared to a nonspecific negative control antibody (red). |
| Background | Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularily in breast cancer (4-9).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Enzyme Analysis: Cathepsin D as an Example
In enzyme analysis, capillary electrophoresis (CE) offers the ease of product separation from the substrate, with the ability to use expensive reagents in microvolumes. In CE, enzymes can be measured either as mass (when they are present
组织蛋白酶 cathepsin 在各种动物组织的细胞内（特别是溶酶体部分）发现的一类蛋白酶。此名源自希腊语的“消化”，为威尔斯达特（ R． Willstā atter， 1929）命名的。来源于同一种类组织细胞的也是由组织蛋白酶 A． B． C． D． E等多种酶组成。可以根据所作用的代表性底物的种类进行分类，但似乎各自还有数种同工酶。组织蛋白酶 A的代表性底物是 N-苄氧基羰基 -L-谷酰胺 -L-苯丙氨酸，最适 pH约 5.5（ 3— 6），可能是一种羧肽酶，可为二异丙基磷氟酸
Analysis of Protein Complexes by 2D Blue Native/SDSPAGE and Antibody-Shift Assay
Blue native polyacrylamide gel electrophoresis (BN-PAGE) is a powerful strategy for the separation of native multiprotein complex (MPC). Combined with other techniques, such as SDS–PAGE, immunoblotting, mass spectrometry, and antibody-shift
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